Record Details

Fabrication of phyco-functionalized zinc oxide nanoparticles and their in vitro evaluation against bacteria and cancer cell line

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Fabrication of phyco-functionalized zinc oxide nanoparticles and their in vitro evaluation against bacteria and cancer cell line
 
Creator Ramakrishnan, Geethalakshmi
Razack, Sirajunnisa Abdul
Ravi, Logeswari
Sahadevan, Renganathan
 
Subject Antineoplastic
Bactericide
Co-precipitation
Kappaphycus alvarezii
Nano-agent
 
Description 770-778
Macroalgae with innumerable metabolites are predominantly identified as potential organisms in industrial and
pharmaceutical applications. Herein, an attempt was made to fabricate zinc oxide nanoparticles (ZnO NPs) using the aqueous
extract of red macroalgae Kappaphycus alvarezii and evaluate its anti-tumor and anti-bacterial activities, in vitro. ZnO NPs was
prepared via chemical reduction (C-ZnO NPs), for comparative analysis, and co-precipitation method with the extract (B-ZnO
NPs). Chemical composition analyses, physical characterization by UV Spectrophotometer, Fourier Transform InfraRed (FTIR)
spectroscopy, Energy Dispersive X-Ray Analysis (EDX) and Scanning Electron Microscopy (SEM) and anti-oxidant ability by
DPPH assay were performed. Anti-microbial activity of the fabricated nanomaterials was performed against E. coli, Bacillus
subtilis and Staphylococcus aureus. Cell toxicity study was carried out using MTT assay upon 3T3 and MCF-7 cell lines. The
study resulted that the presence of phenolic content in the algal extract enhanced the biological activities of ZnO NPs at an
optimized concentration of 100 mM. Total phenolic content was exa mined to be 83.73 mg gallic acid eq/g and the maximum
efficiency of DPPH scavenging was revealed by the concentration of 51.99 mg/mL. Anti-bacterial effect of B-ZnO NPs
demonstrated maximum efficiency at the concentrations of 800 μg/mL, 400 μg/mL and 600 μg/mL against Escherichia coli,
Bacillus subtilis and Staphylococcus aureus respectively. The cell viability study denoted that the fabricated B-ZnO NPs were
non-toxic towards 3T3 while significant cytotoxicity was observed against MCF-7 at an IC50 of 75 μg/mL at 48 h. In
conclusion, the B-ZnO NPs was observed to be effective as both bactericidal and anti-cancerous nano-agent and considered
further for pharmaceutical applications.
 
Date 2023-09-27T12:05:17Z
2023-09-27T12:05:17Z
2023-09
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/62603
https://doi.org/10.56042/ijbb.v60i10.397
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJBB Vol.60(10) [October 2023]