Eleutherine bulbosa bulb extract induces apoptosis and inhibits cell migration by downregulating Sonic hedgehog in human tongue cancer cells: An in vitro study
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Title |
Eleutherine bulbosa bulb extract induces apoptosis and inhibits cell migration by downregulating Sonic hedgehog in human tongue cancer cells: An in vitro study
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Creator |
Sandra, Ferry
Suryajaya, Kelvin Chouw, Angliana Celinna, Maria Ranggaini, Dewi Lee, Kyung Hoon Sartika, Cynthia Retna |
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Subject |
Apoptosis
Cell migration Eleutherine bulbosa Sonic hedgehog Tongue cancer |
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Description |
763-769
Eleutherine bulbosa (Mill.) Urb. is a medicinal plant which has long been used to treat cancer. E. bulbosa bulb extract (EBBE) has been reported to show cytotoxicity towards several types of human cancer. However, the cytotoxic effect of EBBE towards tongue cancer cells has not been investigated. The present study aimed to evaluate the effects of EBBE towards the viability, apoptosis, and migratory activities of tongue cancer cells. Human oral squamous cell carcinoma (HSC)-3 cells were treated with various concentrations of EBBE for 24 h. The number of viable and apoptotic HSC-3 cells were measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and sub-G1 assay, respectively. The migratory activities of HSC-3 cells were assessed using scratch and transwell assay. Sonic hedgehog (SHH) expression was measured using immunoblotting. Upon EBBE treatment, apoptotic HSC-3 cells were significantly higher in a concentration-dependent manner (P |
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Date |
2023-09-27T12:09:21Z
2023-09-27T12:09:21Z 2023-09 |
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Type |
Article
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Identifier |
0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/62606 https://doi.org/10.56042/ijbb.v60i10.1293 |
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Language |
en
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Publisher |
NIScPR-CSIR, India
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Source |
IJBB Vol.60(10) [October 2023]
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