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Green synthesis, characterization and anticancer activity of fungal asparaginase from Aspergillus terreus conjugated on MgO-ZnO nanocomposite against liver cancer cells

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Title Green synthesis, characterization and anticancer activity of fungal asparaginase from Aspergillus terreus conjugated on MgO-ZnO nanocomposite against liver cancer cells
 
Creator G, Baskar
AR, Abinesh
A, Sheik Mohammed Raja
K, Keerthana
A, Supriya
R, Pravin
 
Subject Anticancer
Antitumor
Chemotherapy
Conjugation
Horsehound
Mesosphaerum suaveolens
Pignut
Wild spikenard
 
Description 761-766
L-Asparaginase exists as an effective chemotherapeutic drug for various cancer cell types. However, the L-asparaginaseenzyme in its free state is unstable and has reduced half-life period with numerous side effects. Here, as a green synthesis approach, we have tried a nanocarrier to reduce its side effects and thereby the environmental pollution and toxicity. We used leaf extract of Mesosphaerum suaveolens (L.) Kuntze, commonly called Pignut, Wild spikenard or Horsehound, to produce a Mg-ZnO nanocomposite, which has antitumor activity by arresting the cell cycle of tumour cells. To the synthesized nanocomposite, extracted L-asparaginase enzyme from Aspergillus terreus was impregnated. The presence of ZnO helps to preserve the biological activity of immobilized L-asparaginase along with MgO making the nanocarrier biocompatible and biodegradable. This helps in increasing the stability and anticancer property of the L-asparaginase. The enzyme-impregnated nanobiocomposite was characterized using UV-Vis spectroscopy which ensured the existence of the Asp-Mg-ZnO in the nanobiocomposite. The presence of functional group and the crystallinity nature of the nanobiocomposite were studied using FT-IR and XRD, respectively. The morphological structure and elemental composition of the nanobiocomposite were determined using the SEM-EDAX. The anticancer activity of the synthesized Asp-Mg-ZnO nanobiocomposite was determined using the MTT assay, which helped to determine the viability and proliferation capacity of the cancer cells.
 
Date 2023-09-29T11:29:00Z
2023-09-29T11:29:00Z
2023-09
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/62636
https://doi.org/10.56042/ijeb.v61i10.1642
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.61(10) [October 2023]