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Detection of Mycobacterium bovis lymphocyte stimulating antigens in culture filtrates of a recombinant Mycobacterium smegmatis cosmid library

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Title Detection of Mycobacterium bovis lymphocyte stimulating antigens in culture filtrates of a recombinant Mycobacterium smegmatis cosmid library
 
Creator Gormley, E.
Fray, L.
Sandall, L.
Ke, G.
Dupont, C.
Carpenter, E.
 
Subject vaccination
infectious diseases
 
Description Culture filtrates derived from a Mycobacterium bovis cosmid library in Mycobacterium smegmatis were screened for bovine lymphocyte stimulatory antigens using peripheral blood mononuclear cells (PBMC) from cattle vaccinated with a low dose of Mycobacterium bovis BCG. Lymphocyte proliferation and interferon-gamma (IFN-?) production were used as cellular response markers for antigen recognition. In the primary screen, approximately 28% of all culture filtrates (CF) stimulated responses by PBMC from at least two out of four vaccinated cattle. In one of these CF, the M. bovis Ag85-B antigen was detected by Western-blot analysis. Despite heterogeneous lymphocyte responses of the animals, twenty-four of the culture filtrates stimulated lymphocyte proliferation and IFN-? production from at least six out of eight vaccinated animals in a secondary screen. Analysis of the cosmid DNA associated with these positive CF demonstrated that several contained homologous DNA sequences. It appears that the lymphocyte screening has detected M. bovis antigens that are immuno-dominant in cattle vaccinated with M. bovis BCG.
 
Date 1999-07-16
2014-04-14T10:56:05Z
2014-04-14T10:56:05Z
 
Type Journal Article
 
Identifier Gormley, E., Fray, L., Sandall, L., Ke, G., Dupont, C. and Carpenter, E. 1999. Detection of Mycobacterium bovis lymphocyte stimulating antigens in culture filtrates of a recombinant Mycobacterium smegmatis cosmid library. Vaccine 17(22): 2792-2801.
0264-410X
https://hdl.handle.net/10568/35345
https://doi.org/10.1016/S0264-410X(99)00091-2
 
Rights Copyrighted; all rights reserved
Limited Access
 
Format p. 2792-2801
 
Publisher Elsevier BV
 
Source Vaccine