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Characterisation of single domain ATP-binding cassette protein homologues of Theileria parva

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Title Characterisation of single domain ATP-binding cassette protein homologues of Theileria parva
 
Creator Kibe, M.K.
Macklin, M.D.
Gobright, E.I.
Bishop, Richard P.
Urakawa, T.
Ole-MoiYoi, O.K.
 
Subject theileria parva
genes
dna
rna
immunization
chromosomes
electrophoresis
polymorphism
isolation
infectious diseases
parasitology
 
Description Two distinct genes encoding single domain, ATP-binding cassette transport protein homologues of Theileria parva were cloned and sequenced. Neither of the genes is tandemly duplicated. One gene, TpABCI, encodes a predicted protein of 593 amino acids with an N-terminal hydrophobic domain containing six potential membrane-spanning segments. A single discontinuous ATP-binding element was located in the C-terminal region of TpABCI. The second gene, TpABC2, also contains a single C-terminal ATP-binding motif. Copies of TpABC2 were present at four loci in the T. parva genome on three different chromosomes. TpABCI exhibited allelic polymorphism between stocks of the parasite. Comparison of cDNA and genomic sequences revealed that TpABCl contained seven short introns, between 29 and 84 bp in length. The full-length TpABCI protein was expressed in insect cells using the baculovirus system. Application of antibodies raised against the recombinant antigen to western blots of T. parva piro-plasm lysates detected an 85 kDa protein in this life-cycle stage.
 
Date 2001-09-01
2013-07-03T05:26:03Z
2013-07-03T05:26:03Z
 
Type Journal Article
 
Identifier Parasitology Research;87: 741-750
0932-0113
https://hdl.handle.net/10568/33101
https://doi.org/10.1007/s004360100433
 
Language en
 
Rights Copyrighted; all rights reserved
Limited Access
 
Format p. 741-750
 
Publisher Springer Science and Business Media LLC
 
Source Parasitology Research