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Phenotypic characterization and validation of provitamin A functional genes in early maturing provitamin A-quality protein maize (Zea mays) inbred lines

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Title Phenotypic characterization and validation of provitamin A functional genes in early maturing provitamin A-quality protein maize (Zea mays) inbred lines
 
Creator Obeng‐Bio, E.
Badu-Apraku, Baffour
Ifie, B.E.
Danquah, A.
Blay, E.T.
Dadzie, M.A.
 
Subject genes
maize
maturity
proteins
inbred lines
provitamins
drought stress
genetics
 
Description The number of drought and low‐N tolerant hybrids with elevated levels of provitamin A (PVA) in sub‐Saharan Africa could increase when PVA genes are optimized and validated for developed drought and low‐N tolerant inbred lines. This study aimed to (a) determine the levels of drought and low‐N tolerance, and PVA concentrations in early maturing PVA‐quality protein maize (QPM) inbred lines, and (b) identify lines harbouring the crtRB1 and LcyE genes as sources of favourable alleles of PVA. Seventy early maturing PVA‐QPM inbreds were evaluated under drought, low‐N and optimal environments in Nigeria for two years. The inbreds were assayed for PVA levels and the presence of PVA genes using allele‐specific PCR markers. Moderate range of PVA contents was observed for the inbreds. Nonetheless, TZEIORQ 55 combined high PVA concentration with drought and low‐N tolerance. The crtRB1‐3′TE primer and the KASP SNP (snpZM0015) consistently identified nine inbreds including TZEIORQ 55 harbouring the favourable alleles of the crtRB1 gene. These inbreds could serve as donor parents of the favourable crtRB1‐3′TE allele for PVA breeding in maize.
 
Date 2020-06
2020-07-23T13:55:50Z
2020-07-23T13:55:50Z
 
Type Journal Article
 
Identifier Obeng‐Bio, E., Badu‐Apraku, B., Ifie, B.E., Danquah, A., Blay, E.T. & Dadzie, M.A. (2019). Phenotypic characterization and validation of provitamin A functional genes in early maturing provitamin A‐quality protein maize (Zea mays) inbred lines. Plant Breeding, 1-14.
0179-9541
https://hdl.handle.net/10568/108858
https://doi.org/10.1111/pbr.12798
BIOTECH & PLANT BREEDING
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format p. 575-588
application/pdf
 
Publisher Wiley
 
Source Plant Breeding