Record Details


Field	Value

Title	Homologous minicircles in Leishmania donovani

Creator	Lambson, B. Barker, D.C.

Subject	livestock research infectious diseases parasitology

Description	Leishmania minicircular deoxyribonucleic acid (DNA) is arranged into different classes according to sequence. These classes differ substantially in sequence, despite species- and genus-specific regions, and are present in widely different copy numbers within and between Leishmania strains. Homologous minicircles have been identified in different species of Leishmania by comparing sequences of known minicircles. However, it is possible to select for minicircles of the same class by amplifying Leishmania DNA with polymerase chain reaction primers from the conserved and variable regions. This approach was used with 2 different minicircle classes in the L. donovani complex. In all isolates tested it was possible to amplify minicircles of the selected class.

Date	2002-04 2014-04-14T10:55:42Z 2014-04-14T10:55:42Z

Type	Journal Article

Identifier	Lambson, B. and Barker, D.C. 2002. Homologous minicircles in Leishmania donovani. Transactions of the Royal Society of Tropical Medicine and Hygiene 96(S1): 75-80 359203 https://hdl.handle.net/10568/35262 https://doi.org/10.1016/S0035-9203(02)90055-6

Rights	Limited Access

Format	p. S75-S80

Publisher	Oxford University Press (OUP)

Source	Transactions of the Royal Society of Tropical Medicine and Hygiene

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