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Endophytic fungi improve management of the burrowing nematode in banana (Musa spp.) through enhanced expression of defence-related genes

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Title Endophytic fungi improve management of the burrowing nematode in banana (Musa spp.) through enhanced expression of defence-related genes
 
Creator Kisaakye, J.
Fourie, H.
Coyne, D.
Cortada, L.
Khamis, F.M.
Subramanian, S.
Masinde, S.
Haukeland, S.
 
Subject biological control
endophytes
gene expression
radopholus similis
tissue culture
bananas
 
Description Open Access Article
The root-burrowing nematode, Radopholus similis, is reputedly the most damaging nematode pest of banana and responsible for major production losses. In this study, the endophytic potential of 13 fungal isolates was assessed for the management of R. similis in East African Highland bananas (‘Ng’ombe’). All isolates successfully colonised tissue-cultured banana roots, with isolates from Trichoderma, Fusarium and Hypocrea producing the highest (⩾49.1%) and Beauveria isolates the lowest (⩽14.4%) colonisation. The fungal endophytes T. asperellum (ICIPE 700) and H. lixii (ICIPE 697) were the most effective in reducing R. similis densities (>81%) relative to the non-inoculated control. However, the combined inoculation of ICIPE 700 and ICIPE 697 led to greater suppression of R. similis (>21%) relative to individual inoculation. Suppression of R. similis following inoculation of banana roots with ICIPE 700 and/or ICIPE 697 was associated with the significant upregulation of the defence-related gene PR-1, the cell signalling gene calmodulin Ca2+ and the cell-wall-strengthening gene β-1,3-glucan synthase. This study demonstrates the potential for nematode management in bananas with fungal endophytes, especially using the isolates ICIPE 700 and ICIPE 697 when combined.
 
Date 2023-03-02
2023-08-01T09:53:48Z
2023-08-01T09:53:48Z
 
Type Journal Article
 
Identifier Kisaakye, J., Fourie, H., Coyne, D., Cortada, L., Khamis, F.M., Subramanian, S., ... & Haukeland, S. (2023). Endophytic fungi improve management of the burrowing nematode in banana (Musa spp.) through enhanced expression of defence-related genes. Nematology, 25(4), 427-442.
1388-5545
https://hdl.handle.net/10568/131364
https://doi.org/10.1163/15685411-bja10229
NATURAL RESOURCE MANAGEMENT
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format p. 427-442
application/pdf
 
Publisher Brill
 
Source Nematology