Record Details

Provitamin A accumulation in Cassava (Manihot esculenta) roots driven by a single nucleotide polymorphism in a phytoene synthase gene[W]

CGSpace

View Archive Info
 
 
Field Value
 
Title Provitamin A accumulation in Cassava (Manihot esculenta) roots driven by a single nucleotide polymorphism in a phytoene synthase gene[W]
 
Creator Welsch, R
Arango, Jacobo
Bar, C
Salazar, B.
Al-Babili, S.
Beltrán Giraldo, Jorge Alonso
Chavarriaga Aguirre, Paul
Ceballos, H.
Tohme, Joseph M.
Beyer, Peter
 
Subject manihot esculenta
varieties
vitamins
carotenoids
plant breeding
polymorphism
retinol
roots
variedades
vitaminas
carotenoides
mejoramiento de plantas
polimorfismo
raíces
cell biology
 
Description Cassava (Manihot esculenta) is an important staple crop, especially in the arid tropics. Because roots of commercial cassava cultivars contain a limited amount of provitamin A carotenoids, both conventional breeding and genetic modification are being applied to increase their production and accumulation to fight vitamin A deficiency disorders. We show here that an allelic polymorphism in one of the two expressed phytoene synthase (PSY) genes is capable of enhancing the flux of carbon through carotenogenesis, thus leading to the accumulation of colored provitamin A carotenoids in storage roots. A single nucleotide polymorphism present only in yellow-rooted cultivars cosegregates with colored roots in a breeding pedigree. The resulting amino acid exchange in a highly conserved region of PSY provides increased catalytic activity in vitro and is able to increase carotenoid production in recombinant yeast and Escherichia coli cells. Consequently, cassava plants overexpressing a PSY transgene produce yellow-fleshed, high-carotenoid roots. This newly characterized PSY allele provides means to improve cassava provitamin A content in cassava roots through both breeding and genetic modification.
 
Date 2010-11-24
2014-10-02T08:33:03Z
2014-10-02T08:33:03Z
 
Type Journal Article
 
Identifier 1532-298X
1040-4651
https://hdl.handle.net/10568/43980
http://www.plantcell.org/content/22/10/3348.short
https://doi.org/10.1105/tpc.110.077560
 
Language en
 
Rights Open Access
 
Publisher Oxford University Press (OUP)
 
Source Plant Cell