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Antimicrobial activity of Mystroxylon aethiopicum leave extracts and isolated 3-O-acetyloleanolic acid

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Title Antimicrobial activity of Mystroxylon aethiopicum leave extracts and isolated 3-O-acetyloleanolic acid
 
Creator Malada, Mutshidzi P
Mazimba, Ofentse
Mogashoa, Mary M
Masoko, Peter
 
Subject Antibacterial activity
Minimum inhibitory concentration
Mystroxylon aethiopicum
3-O-acetyloleanolic acid
 
Description 464-469
Mystroxylon aethiopicum (Thumb.) Loes subsp. schlechteri (loes.) R. H. Archeris, a member of the Celastracea family,
is widely used in traditional medicine to treat several diseases, such as hemorrhagic diarrhoea and respiratory tract
infections. The study aimed to isolate the biologically active compounds from the leaves of M. aethiopicum. The leaves
were ground to a fine powder and sequentially extracted with n-hexane, dichloromethane, acetone and methanol using serial
exhaustive extraction (SEE) method. The bioautography and broth micro-dilution methods were used to analyse the
antibacterial activities. The bioautography assay revealed that the compounds in the n-hexane, dichloromethane and acetone
extracts have activity against all tested microorganisms. In contrast, the methanol extracts exhibited no antibacterial activity
against Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and Escherichia coli. The n-hexane extract
had the lowest average MIC of 0.08 mg/mL, followed by the acetone (0.71 mg/mL) and dichloromethane (0.82 mg/mL)
extracts. The bioassay-guided fractionation of dichloromethane and acetone extracts led to the isolation of 3-Oacetyloleanolic
acid. The structure of the isolated compound was elucidated using NMR data and comparison with literature
data. This is the first report on the isolation of 3-O-acetyloleanolic acid from the leaves of M. aethiopicum.
 
Date 2023-10-26T06:49:53Z
2023-10-26T06:49:53Z
2023-09
 
Type Article
 
Identifier 0976-0512 (Online); 0976-0504 (Print)
http://nopr.niscpr.res.in/handle/123456789/62801
https://doi.org/10.56042/ijnpr.v14i3.5520
 
Language en
 
Relation Int. cl. (2021.01)− A61K 36/00, A61K 36/37, A61K 127/00, A61P 31/00
 
Publisher NIScPR-CSIR, India
 
Source IJNPR Vol.14(3) [September 2023]