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Identification of Novel QTLs/Defense Genes in Spring Wheat Germplasm Panel for Seedling and Adult Plant Resistance to Stem Rust and Their Validation Through KASP Marker Assays

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Relation http://oar.icrisat.org/12244/
https://apsjournals.apsnet.org/doi/abs/10.1094/PDIS-09-22-2242-RE
https://doi.org/10.1094/PDIS-09-22-2242-RE
 
Title Identification of Novel QTLs/Defense Genes in Spring Wheat Germplasm Panel for Seedling and Adult Plant Resistance to Stem Rust and Their Validation Through KASP Marker Assays
 
Creator Pradhan, A K
Budhlakoti, N
Mishra, D C
Prasad, P
Bhardwaj, S C
Sareen, S
Sivasamy, M
Jayaprakash, P
Geetha, M
Nisha, R
Shajitha, P
Peter, J
Kaur, A
Kaur, S
Vikas, V K
Singh, K
Kumar, S
 
Subject Plant Genetics
Wheat
Germplasm
 
Description Stem rust is one of the major diseases threatening wheat production globally. To identify novel resistance quantitative trait loci (QTLs), we performed 35K Axiom Array SNP genotyping assays on an association mapping panel of 400 germplasm accessions, including Indian landraces, in conjunction with phenotyping for stem rust at seedling and adult plant stages. Association analyses using three genome wide association study (GWAS) models (CMLM, MLMM, and FarmCPU) revealed 20 reliable QTLs for seedling and adult plant resistance. Among these 20 QTLs, five QTLs were found consistent with three models, i.e., four QTLs on chromosome 2AL, 2BL, 2DL, and 3BL for seedling resistance and one QTL on chromosome 7DS for adult plant resistance. Further, we identified a total of 21 potential candidate genes underlying QTLs using gene ontology analysis, including a leucine rich repeat receptor (LRR) and P-loop nucleoside triphosphate hydrolase, which have a role in pathogen recognition and disease resistance. Furthermore, four QTLs (Qsr.nbpgr-3B_11, QSr.nbpgr-6AS_11, QSr.nbpgr-2AL_117-6, and QSr.nbpgr-7BS_APR) were validated through KASP located on chromosomes 3B, 6A, 2A, and 7B. Out of these QTLs, Sr.nbpgr-7BS_APR was identified as a novel QTL for stem rust resistance which has been found effective in both seedling as well as the adult plant stages. Identified novel genomic regions and validated QTLs have the potential to be deployed in wheat improvement programs to develop disease resistant varieties for stem rust and can diversify the genetic basis of resistance.
 
Publisher The American Phytopathological Society (APS)
 
Date 2023-06-13
 
Type Article
PeerReviewed
 
Identifier Pradhan, A K and Budhlakoti, N and Mishra, D C and Prasad, P and Bhardwaj, S C and Sareen, S and Sivasamy, M and Jayaprakash, P and Geetha, M and Nisha, R and Shajitha, P and Peter, J and Kaur, A and Kaur, S and Vikas, V K and Singh, K and Kumar, S (2023) Identification of Novel QTLs/Defense Genes in Spring Wheat Germplasm Panel for Seedling and Adult Plant Resistance to Stem Rust and Their Validation Through KASP Marker Assays. Plant Disease, 107 (6). pp. 1847-1860. ISSN 1943-7692