Evaluation of bioactive potentials in the crude flavonoid fraction from the leaves of Butea monosperma (Lam.)Taub. in vitro and in vivo in experimental models
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Title |
Evaluation of bioactive potentials in the crude flavonoid fraction from the leaves of Butea monosperma (Lam.)Taub. in vitro and in vivo in experimental models
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Creator |
Bala, Rajni
Mehta, Sakshi Roy, Vikas C Roy, Ram C Roy, Sharda Singh, Jaspreet |
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Subject |
Anticonvulsant
Dhak Epilepsy Antioxidants Forest flame Maximal electroshock Oxidative stress Palash Pentylene tetrazole Sacre tree Seizures |
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Description |
893-898
Oxidative stress is responsible for the advent of seizure hence, antioxidants are used for the treatment neurodegenerative ailments such as epilepsy. The Sacred tree, Butea monosperma (Lam.)Taub. is known to possess several medicinal properties due to the presence of steroids, alkaloids, triterpenoids, polyphenols, flavonoids, tannins and glycosides, particularly in the leaves. In the present study, we evaluated the bioactive potentials in the isolated crude flavonoid fraction (FF) from its leaves. FF was isolated by successive methanol extract method and was evaluated for antioxidant and anticonvulsant activities in vitro and in vivo. For in vivo study, 25 healthy Wistar albino rats were employed in five groups: Gr.I: control; Gr. II: standard group receiving phenytoin sodium; and Gr. III-V: test groups receiving oral doses of FF i.e., 50, 100 and 200 mg/kg, respectively. In vitro data showed concentration dependent antioxidant activity (88.86±0.006%), reducing power activity (0.694±0.002%) and nitric oxide scavenging activity (87.98±0.013%). In vivo, a significant depression in the phases and percentage incidence of convulsions was observed in the maximal electroshock model in a dose-dependent manner. The pentylene tetrazole-induced seizure model also depicted a significant (P ≤0.001) decrease in the latency of convulsions and an increase in the percentage protection against the convulsions in a dose-dependent manner. Our findings suggest antioxidant and anticonvulsant potential of FF obtained from Butea monosperma leaves. |
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Date |
2023-11-01T07:10:05Z
2023-11-01T07:10:05Z 2023-11 |
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Type |
Article
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Identifier |
0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/62845 https://doi.org/10.56042/ijeb.v61i11.1533 |
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Language |
en
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Publisher |
NIScPR-CSIR, India
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Source |
IJEB Vol.61(11) [November 2023]
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