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Role of microRNA-16-5p, microRNA-194, IP-10 and APRIL in inducing inflammation in SARS-CoV-2 infected patients with severe symptoms

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Title Role of microRNA-16-5p, microRNA-194, IP-10 and APRIL in inducing inflammation in SARS-CoV-2 infected patients with severe symptoms
 
Creator Sarrafi, Omidreza
Kariminik, Ashraf
Arababadi, Mohammad Kazemi
 
Subject COVID-19
Humoral immunity
 
Description 888-892
The immune system induces pro-inflammatory conditions in the hospitalized SARS-CoV-2 infected patients. The roles
played by humoral immunity-related factors in the pro-inflammatory conditions of the patients are yet to be clarified.
It has been revealed that a proliferation-inducing ligand (APRIL), interferon (IFN)-γ-inducible protein 10 (IP-10) and
microRNA-16-5p (miR-16-5p) play key roles in the induction of inflammation. Thus, in this study, we explored the
expression levels of APRIL, IP-10, miR16-5p and miR-194 in the SARS-CoV-2 infected patients with severe symptoms. In
addition, miR-194 can inhibit immune responses against viral infection. Further, we evaluated the expression of the
molecule in the patients to explore the effect of the molecule during coronavirus disease 2019 (COVID-19). About 60 severe
SARS-CoV-2 infected patients who were in the peak of the disease and 60 healthy controls were enrolled to evaluate
APRIL, IP-10, miR16-5p and miR-194 expression levels. IP-10 expressions were evaluated using enzyme linked
immunoassay (ELISA), while APRIL, miR-16-5p and miR-194 were evaluated using Real-Time PCR technique. The results
showed that APRIL, miR-16-5p and miR-194 expression and serum levels of IP-10 significantly increased in the
hospitalized SARS-CoV-2 infected patients compared to the healthy controls. There was a positive correlation between
miR-16-5p and miR-194 expression levels in the patients. The significant participation of miR-16-5p in the induction of
inflammation indicates its key role along with APRIL and IP-10 for excess inflammation in the hospitalized SARS-CoV-2
infected patients with severe symptoms. Upregulation of miR-194 may be natural negative feedback to the pro-inflammatory
conditions and may be associated with establishment of SARS-CoV-2 infection.
 
Date 2023-11-01T07:11:38Z
2023-11-01T07:11:38Z
2023-11
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/62846
https://doi.org/10.56042/ijeb.v61i11.1922
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.61(11) [November 2023]