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Protective effect of Diospyros kaki L. leaves extract against oxidative damage and inflammation in the testicular ischemia-reperfusion model in rats

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Title Protective effect of Diospyros kaki L. leaves extract against oxidative damage and inflammation in the testicular ischemia-reperfusion model in rats
 
Creator Yurtal, Ziya
Kulualp, Kadri
Özkan, Hüseyin
Kutlu, Tuncer
Alakuş, Halil
Aslan, Abdullah
Altuğ, Muhammed E.
Güvenç, Mehmet
Kaya, Ufuk
 
Subject Chinese persimmon
Japanese persimmon
Oriental persimmon
Reactive oxygen species (ROS)
Testicular torsion
 
Description 871-880
The Oriental persimmon, Diospyros kaki L., native of China, with its bioactive compounds from leaves, flavonoids and
polyphenols in particular, possess antibacterial, antiallergic, anti-inflammatory, neuroprotective and antihypertensive
properties. Though, the anti-inflammatory and antioxidative effects of the D. kaki leaves have beneficial effects in I/R injury
in the brain, the same has not been investigated in testicular I/R injury until now. Hence, in the present study,
we investigated its leaf extract against oxidative damage and inflammation in the testicular ischemia-reperfusion model.
Thirty-two male rats were randomly and equally divided into four groups. While saline (SF) was given to the control and
ischemia/reperfusion (I/R) groups for 5 days, Diospyros kaki (DK) and I/R+DK were given 200 mg/kg of DK leaf extract.
Afterwards, left orchiectomy was performed in control and DK, and testicular torsion was performed in I/R and I/R+DK for
2 hours. All rats were sacrificed 24 hours after procedures and samples were collected. Compared to the control, I/R had
high MDA levels, low GSH and GSH-Px levels; compared to the I/R, MDA levels of the I/R+DK were lower, and the GSH
and GSH-Px levels were higher. In I/R, the presence of congestion and hemorrhage were determined and the deterioration in
seminiferous tubule structure and spermatogenesis were less in the I/R+DK. TNF-α, COX-2, BCL-2, and BAX genes
expressions were significantly lower in the I/R+DK group. TNF-α and COX-2 proteins were higher in I/R and I/R+DK
groups, while NRF2 was higher in I/R+DK. These results show that DK leaves extract has crucial protective effect against
oxidative damage and inflammation in the I/R model of rats.
 
Date 2023-11-01T07:20:13Z
2023-11-01T07:20:13Z
2023-11
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/62848
https://doi.org/10.56042/ijeb.v61i09.3638
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.61(11) [November 2023]