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Effect of gonadotropins on blood, bone marrow and spleen in cyclophosphamide exposed rats

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Title Effect of gonadotropins on blood, bone marrow and spleen in cyclophosphamide exposed rats
 
Creator Koluman, Başak Ünver
Çil, Nazlı
Mete, Gülçin Abban
 
Subject Cytotoxic chemotherapy
Drug toxicity
 
Description 861-870
Cyclophosphamide (CTX) is an effective chemotherapeutic agent. Gonadotropins are molecules with various actions. Here, we investigated the effects of gonadotropins on the peripheral blood, bone marrow and spleen in rats administered with CTX. Three groups were formed: Control (C) group with no process; Sham (S) group: Physiological saline was applied; CTX group: A single dose of 200 mg/kg and 8 mg/kg CTX was administered for the next 14 days. All rats were superovulated with 150-300 IU/kg pregnant mare serum gonadotropin. Human chorionic gonadotropin @150-300 IU/kg was given, and complete blood counts, bone marrow smears, and spleen sections were examined; and also the expression of WNT-1, WNT-4, and β-catenin was analyzed. Although the hemoglobin and platelet value in the CTX group was lowest, it was still within the normal reference ranges. The C and S groups had significantly higher white blood cell values (p=0.017). In terms of number of megakaryocytes, Myeloid/ Erythroid ratio, lymphoid cell ratios, no significant differences were found in bone marrow aspiration smears. The CTX group had significantly higher β-catenin expression in the red pulp than the other groups (p=0.0001). The CTX group had the highest WNT-4 expression and very intense expression of WNT-1 in the white pulp. Our results indicate that the gonadotropins, promising in "treatment", have favourable effects on toxicity of CTX.
 
Date 2023-11-01T07:21:36Z
2023-11-01T07:21:36Z
2023-11
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/62849
https://doi.org/10.56042/ijeb.v61i11.1764
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.61(11) [November 2023]