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Apoptotic and antiproliferative effects of Urtica dioica L. extract on K562 chronic myeloid leukemia cell line

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Title Apoptotic and antiproliferative effects of Urtica dioica L. extract on K562 chronic myeloid leukemia cell line
 
Creator Sevinc, Sevgi Kocyigit
Karadeniz, Melike
Sen, Ali
Orun, Oya
Göger, Fatih
Bagatur, İbrahim Hulusi
Olgac, Ercan
Kırmacı, Mahmut Ramazan
Tuyan, Mustafa Emre
Yalman, Umut
Tiber, Pinar Mega
 
Subject Apoptosis
Caffeic acid
Cancer
Chlorogenic acid
Malic acid
Rutin
Stinging nettle
 
Description 833-842
Urtica dioica L. (stinging nettle) is an herb commonly used as a medical supplement for its anticarcinogenic,
antiinflammatory, analgesic, antifungal, and antioxidant effects. In this study, we analyzed phytochemical composition of
Urtica dioica ethanol extract and investigated its apoptotic and antiproliferative effects in K562 cell line, a model cell line
for chronic myeloid leukemia (CML). K562 cells exposed to different concentrations of U. dioica extract (0, 25, 50, 75 and
100 g/mL) were tested for cell proliferation and apoptosis using appropriate methods and different phases of the cell cycle
were analyzed using flow cytometry. Phenolic content of the plant was analyzed by LC–MS/MS. We showed that U. dioica
ethanol extract has four phenolic compounds: caffeic acid, chlorogenic acid (major compound), malic acid and rutin. Urtica
dioica significantly repressed proliferation of K562 cells compared to control mononuclear cells (PBMCs) isolated from the
peripheral blood of healthy donors. An increase in Bax/Bcl-2 and PARP cleavage ratio, decrease in mitochondrial
membrane potential indicated that the extract inhibited cell proliferation through an apoptosis-mediated process.
Furthermore, cell cycle arrest at G0/G1 phase increased in a concentration dependent manner. Our results explicitly
demonstrate that ethanol extract of Urtica dioica leads to a decrease in cell viability and proliferation, initiates apoptosis,
and induces G0/G1 cell cycle accumulation in this cell line. This is the first study to have demonstrated the antiproliferative
and apoptotic effects of Urtica dioica on chronic myeloid leukemia (CML) cell lines K562 in vitro.
 
Date 2023-11-01T08:49:13Z
2023-11-01T08:49:13Z
2023-11
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/62852
https://doi.org/10.56042/ijeb.v61i09.3638
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.61(11) [November 2023]