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Antiproliferative potential of bioactive molecule from Murraya koenigii L. Spreng against breast cancer: In vitro and in vivo studies and gene expression analyses

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Title Antiproliferative potential of bioactive molecule from Murraya koenigii L. Spreng against breast cancer: In vitro and in vivo studies and gene expression analyses
 
Creator Ramamoorthy, Mullai Valli
Prashant, Bhamare Deepak
Mathew, Deepu
Devassy, Babu Thekkekara
Raman, Shylaja Muthangaparambil
Muhammed, Pareeth Chennattu
Nair, Smita
 
Subject Antioxidant fraction
Drug design
Mammary tumour model
Molecular docking
Oncogene expression
 
Description 819-832
The curry leaf extract is known to have anticancer property against breast cancer. Identification of the specific compound
therein the curry leaf and its validation is essential for successful discovery of drugs. In that content, here, we extracted
oleoresin from the mature curry leaves was subjected to antioxidant fractionation using column chromatography. Fraction
obtained using 60:40 hexane and ethyl acetate solvent system, showing the maximum inhibition of DPPH, was subfractionated
and those with the highest antioxidant property was analyzed in LC-MS/MS. Spectrum of molecules identified,
along with FDA approved drugs, were docked with target proteins for breast cancer. In vitro screening of candidate
phytocompounds doxylamine, histidinol and pheniramine, in their commercially available form, through Trypan blue
exclusion assay against murine cancer cell lines EAC and DLA had shown that they have no cytotoxicity. Pheniramine
maleate salt (PMS), doxylamine succinate salt (DSS) and L-histidinol dihydrochloride (LHD) have shown dose-dependent
inhibition of proliferation of MCF-7 cells, with 280 μg/mL PMS at 72 h of incubation giving the maximum of 98.46%.
Acute toxicity studies in Swiss albino mice (100 mg PMS/kg body wt.) have confirmed that the drug has no toxicity. Mouse
mammary pad tumour model has shown that PMS significantly reduces the WBC count in the tumour induced mice. Liver
function tests, histopathological analyses of liver, mammary pad and kidney tissues and expression analysis of oncogenes
ER-α1, Bcl-2, c-Myc and Pin1 have confirmed the drug candidature of PMS.
 
Date 2023-11-01T08:53:54Z
2023-11-01T08:53:54Z
2023-11
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/62853
https://doi.org/10.56042/ijeb.v61i11.3267
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.61(11) [November 2023]