Record Details

Regulatory role of TRPM7 cation channels on neuronal hypoxia model

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Regulatory role of TRPM7 cation channels on neuronal hypoxia model
 
Creator ÖZ, Ahmi
 
Subject Apoptosis
Calcium signaling
Hypoxia
SH-SY5Y cells
TRPM7 channels
 
Description 836-843
Low levels of oxygen have harmful effects on cells especially in neurons because of their vulnerable status for oxygen
consumption. Intracellular calcium concentration ([Ca2+]i) is accumulated by several kinds of calcium-permeable channels
including the Transient Receptor Potential (TRP) channels. The TRPM7 cation channels are calcium ion (Ca2+) permeable
non-selective cation channels belonging to TRP superfamily. The TRPM7 is expressed in different organs of the human
body including nervous system components especially in the brain. Some of the TRP channel subtypes are related to
oxidative stress and increased oxidative stress triggers channel activity. Recently, TRPM7 cation channels involved in
hypoxia. Hence, alterations of [Ca2+]i may be a key factor inTRPM7activity in hypoxia and preventing hypoxic injury of
neurons TRPM7 could be a therapeutic target. Therefore, it has been investigated effects of downregulation of TRPM7
cation channels by siRNA applications to induce getting TRPM7 activity down. The role of silencing of TRPM7 cation
channels on apoptosis and cell morphology, production of intracellular reactive oxygen species (iROS), mitochondrial
membrane depolarization (MMD) levels, enzymatic activity values of caspase 3, 8 and 9 in SH-SY5Y neuronal cells
investigated in this study. It has been shown that the downregulation of TRPM7 cation channels may prevent cell death and
protect cellular morphology and viability in neuronal cells after chemical hypoxia induction. Decreasing TRPM7 channel
activity may also decrease calcium overload and it is a key regulatory function of TRPM7 channels in hypoxic conditions. In
conclusion, TRPM7 cation channel antagonists or suppression of the channel expression by genetic manipulations can be a
useful and potential therapeutic approach against neuronal hypoxia-related cell death.
 
Date 2023-11-10T07:34:23Z
2023-11-10T07:34:23Z
2023-11
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://nopr.niscpr.res.in/handle/123456789/62894
https://doi.org/10.56042/ijbb.v60i11.4467
 
Language en
 
Publisher NIScPR-CSIR,India
 
Source IJBB Vol.60(11) [November 2023]