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Sui generis development of molecular markers from transcriptome of Crotalaria juncea, a fabaceae family bast fiber crop

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Title Sui generis development of molecular markers from transcriptome of Crotalaria juncea, a fabaceae family bast fiber crop
Not Available
 
Creator D. Saha
A.K. Shaw
P. Mandal
M.R. Thimmaiaha
S. Datta
P. Satya
J. Mitra
G. Kar
 
Subject Bast Fibre
Molecular markers
Intron length Polymorphism
Simple Sequence Repeat
Single nucleotide polymorphism
Sunn hemp
 
Description Not Available
Lack of genomic resources in sunn hemp (Crotalaria juncea L.), a leguminous bast fiber crop, is one of the major constraints for germplasm characterization and genetic analysis. We identified non-redundant sets of a variety of molecular markers from the de novo flower-bud transcriptome of sunn hemp. These molecular markers included 1683 simple sequence repeats, 4759 intron length polymorphism markers primarily from protein-coding genes, and 3309 single nucleotide polymorphism allele-specific markers. All of these marker resources have been integrated into a simple database for easy access. To evaluate marker efficiency and polymorphism information content of the sampled primers, a subset of SSR and intron length polymorphism markers were validated in sunn hemp germplasm accessions to obtain average PIC values of 0.26–0.32. The SSR genotyping profiles also aided in the genetic distance matrix-based clustering of sunn hemp germplasm accessions. The ability of these molecular markers to successfully amplify in cross-genera bast fiber crops further demonstrated their effectiveness in genotyping. Thus, the current study describes the identification of previously undisclosed endogenous molecular markers in sunn hemp, which have potential applications in genetic studies and breeding in sunn hemp for bast fiber improvement.
Not Available
 
Date 2023-10-26T14:35:25Z
2023-10-26T14:35:25Z
2022-11-02
 
Type Journal
 
Identifier Saha, D. Shaw, A.K., Mandal, P., Thimmaiah, M.R., Datta, S., Satya, P., Mitra, J., Kar, G. (2023). Sui generis development of molecular markers from transcriptome of Crotalaria juncea, a fabaceae family bast fiber crop. Journal of Natural Fibres. 20(1): 2139327. https://doi.org/10.1080/15440478.2022.2139327
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http://krishi.icar.gov.in/jspui/handle/123456789/80834
 
Language English
 
Relation Not Available;
 
Publisher Taylor and Francis