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Exploration of urease-mediated biomineralization for defluoridation by Proteus columbae MLN9 with an emphasis on its genomic characterization

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Title Exploration of urease-mediated biomineralization for defluoridation by Proteus columbae MLN9 with an emphasis on its genomic characterization
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Creator M. Let
K. Majhi
U. Halder
A. De
D. Saha
A. Chitikineni
T. Roychowdhury
R.K. Varshney
R. Bandopadhyay
 
Subject Biomineralization
Defluoridation
Fluoride resistant
Genomics
Urease
 
Description Not Available
Fluoride (F-) contamination of groundwater is a silent killer of human health that has now become a global problem. In the present study, we sought to isolate fluoride-resistant, urease producing bacteria from F- contaminated groundwater to evaluate their potential F- biomineralization. Strain MLN9, isolated from the fluoride-contaminated village of Madhabpur in Birbhum, could resist up to 5600 mg L−1 F- concentration. Taxonomic classification of strain MLN9 based on whole genome sequence identified it as Proteus columbae MLN9. Strain MLN9 showed 88.9% of maximum F- removal efficiency at pH 8.0, 1.0 g L−1 CaCl2, and 10.0 g L−1 urea concentration. Scanning electron micrographs showed dense and porous biological crystal precipitates surrounding the surface of the bacterial cells, and the adherence of F- to the cell surface was confirmed by the energy dispersion spectrum. Moreover, the X-ray diffraction pattern showed that the F- precipitates on the cell surface were biological crystals of Ca5(PO4)3F and CaF2. Genomic analysis of strain MLN9 revealed the presence of crcB gene homologs possibly encoding F- transporters and urease regulatory proteins such as UreA, UreB, UreC, UreD, and UreG suggesting a role in defluoridation. Our findings provide new insights into the urease based MICP technology using a noble bacterium Proteus columbae MLN9 for fluoride removal.
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Date 2023-10-26T14:32:58Z
2023-10-26T14:32:58Z
2023-06-01
 
Type Journal
 
Identifier Let, M., Majhi, K., Halder, U., De, A., Saha, D., et al. (2023). Exploration of urease-mediated biomineralization for defluoridation by Proteus columbae MLN9 with an emphasis on its genomic characterization. Journal of Environmental Chemical Engineering. 11(3): 109791. https://doi.org/10.1016/j.jece.2023.109791
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http://krishi.icar.gov.in/jspui/handle/123456789/80832
 
Language English
 
Relation Not Available;
 
Publisher Elsevier