Exploration of urease-mediated biomineralization for defluoridation by Proteus columbae MLN9 with an emphasis on its genomic characterization
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Title |
Exploration of urease-mediated biomineralization for defluoridation by Proteus columbae MLN9 with an emphasis on its genomic characterization
Not Available |
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Creator |
M. Let
K. Majhi U. Halder A. De D. Saha A. Chitikineni T. Roychowdhury R.K. Varshney R. Bandopadhyay |
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Subject |
Biomineralization
Defluoridation Fluoride resistant Genomics Urease |
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Description |
Not Available
Fluoride (F-) contamination of groundwater is a silent killer of human health that has now become a global problem. In the present study, we sought to isolate fluoride-resistant, urease producing bacteria from F- contaminated groundwater to evaluate their potential F- biomineralization. Strain MLN9, isolated from the fluoride-contaminated village of Madhabpur in Birbhum, could resist up to 5600 mg L−1 F- concentration. Taxonomic classification of strain MLN9 based on whole genome sequence identified it as Proteus columbae MLN9. Strain MLN9 showed 88.9% of maximum F- removal efficiency at pH 8.0, 1.0 g L−1 CaCl2, and 10.0 g L−1 urea concentration. Scanning electron micrographs showed dense and porous biological crystal precipitates surrounding the surface of the bacterial cells, and the adherence of F- to the cell surface was confirmed by the energy dispersion spectrum. Moreover, the X-ray diffraction pattern showed that the F- precipitates on the cell surface were biological crystals of Ca5(PO4)3F and CaF2. Genomic analysis of strain MLN9 revealed the presence of crcB gene homologs possibly encoding F- transporters and urease regulatory proteins such as UreA, UreB, UreC, UreD, and UreG suggesting a role in defluoridation. Our findings provide new insights into the urease based MICP technology using a noble bacterium Proteus columbae MLN9 for fluoride removal. Not Available |
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Date |
2023-10-26T14:32:58Z
2023-10-26T14:32:58Z 2023-06-01 |
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Type |
Journal
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Identifier |
Let, M., Majhi, K., Halder, U., De, A., Saha, D., et al. (2023). Exploration of urease-mediated biomineralization for defluoridation by Proteus columbae MLN9 with an emphasis on its genomic characterization. Journal of Environmental Chemical Engineering. 11(3): 109791. https://doi.org/10.1016/j.jece.2023.109791
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/80832 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Elsevier
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