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Coliphage cocktails for controlling antimicrobial-resistant Escherichia coli: emphasizing polyphage and multihost interactions at different levels of multiplicity of infection

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Title Coliphage cocktails for controlling antimicrobial-resistant Escherichia coli: emphasizing polyphage and multihost interactions at different levels of multiplicity of infection
Not Available
 
Creator Manikantha, B.
Murugadas, V.
Karthika, R.
Joshy, C. G.
Visnuvinayagam, S.
Madhusudana Rao, B.
 
Subject antimicrobial resistance
coliphages
E. coli
phage–host interactions
phage cocktails
polyphage combinations
 
Description Not Available
Aim
This study elucidates the in-vitro bactericidal effectiveness of polyphage cocktail combinations of 2, 4, 6, 8, and 10 individual coliphages against a cocktail of 20 AMR Escherichia coli.

Methods and results
Different polyphage cocktails viz., 45 two-phage combinations, 28 four-phage combinations, 15 six-phage combinations, 6 eight-phage combinations, and 1 ten-phage combination were formulated using a pool of ten coliphages that were isolated from two different geographical locations (East and West coasts of India). The different polyphage cocktails were tested at four different levels of Multiplicity of Infection (MOI) viz., MOI-1, MOI-10, MOI-100, and MOI-1000. All the 2, 4, 6, 8, and 10-phage cocktails were found to be effective in controlling the growth of a cocktail of 20 AMR bacteria when tested at MOI-1000 and MOI-100 but variations in antibacterial activity were observed at lower MOIs of 10 and 1. The ten coliphage cocktail showed lytic activity against 100% of AMR E. coli from farmed brackish water shrimp, 96% of laboratory collection of AMR E. coli, 92% of AMR E. coli from farmed freshwater fish, and 85% of AMR E. coli from market shrimp.

Conclusion
Polyphage cocktails of 2, 4, 6, 8, and 10 coliphages applied at an MOI of 1000 effectively suppressed the growth of antimicrobial-resistant E. coli. The results indicated phage–phage synergy in the lytic activity of several coliphage combinations at higher MOIs of 1000 and 100 while phage–phage antagonism was evidenced at lower MOIs of 10 and 1.
Not Available
 
Date 2023-10-25T17:17:35Z
2023-10-25T17:17:35Z
2023-04
 
Type Research Paper
 
Identifier Manikantha, B., Murugadas, V., Karthika, R., Joshy, C. G., Visnuvinayagam, S. and Madhusudana Rao, B. (2023) Coliphage cocktails for controlling antimicrobial-resistant Escherichia coli: emphasizing polyphage and multihost interactions at different levels of multiplicity of infection. J. Appl. Microbiol. 134(4): lxad055.
1364-5072
http://krishi.icar.gov.in/jspui/handle/123456789/80830
 
Language English
 
Relation Not Available;
 
Publisher Oxford Academic