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Ectopic expression of pigeonpea Orf147 gene imparts partial sterility in Cicer arietinum

OAR@ICRISAT

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Relation http://oar.icrisat.org/12305/
https://www.sciencedirect.com/science/article/abs/pii/S0378111923002135
https://doi.org/10.1016/j.gene.2023.147372
 
Title Ectopic expression of pigeonpea Orf147 gene imparts partial sterility in Cicer arietinum
 
Creator Bhattacharya, J
Reddy, D S
Prasad, K
Nitnavare, R B
Bhatnagar-Mathur, P
Reddy, P S
 
Subject Genetic Engineering
Chickpea
Pigeonpea
 
Description Orf147, a cytotoxic peptide, has been found to cause cytoplasmic male sterility (CMS) in Cajanus cajanifolius (pigeonpea). In our study, Orf147 was introduced into self-pollinating Cicer arietinum (chickpea) using Agrobacterium-mediated transformation for induction of CMS. The stable integration and expression of the transgene has been assessed through PCR and qRT-PCR analysis. In addition, phenotypic sterility analysis has been performed, considering developmental parameters like flower development, pod formation and flower drop. Transgene inheritance analysis demonstrates that out of the five PCR positive events in the T0 generation, two events have segregated according to the Mendelian segregation ratio (3:1) in the T2 generation. Further, pollen viability test using microscopic analysis confirms the induction of partial CMS in transgenic chickpea. The study holds significant value regarding the heterosis of self-pollinating legumes like chickpea. As a part of the prospect, exploring inducible promoters of species-specific or related legumes would be the next step to developing a two-line hybrid system.
 
Publisher Elsevier
 
Date 2023-06-05
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights cc_by_nc_nd
 
Identifier http://oar.icrisat.org/12305/1/Gene_868_1-7_2023.pdf
Bhattacharya, J and Reddy, D S and Prasad, K and Nitnavare, R B and Bhatnagar-Mathur, P and Reddy, P S (2023) Ectopic expression of pigeonpea Orf147 gene imparts partial sterility in Cicer arietinum. Gene, 868. pp. 1-7. ISSN 0378-1119