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Association of root hair length and density with yield-related traits and expression patterns of TaRSL4 underpinning root hair length in spring wheat

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Title Association of root hair length and density with yield-related traits and expression patterns of TaRSL4 underpinning root hair length in spring wheat
 
Creator Maqbool, Saman
Saeed, Fatima
Raza, Ali
Rasheed, Awais
Zhonghu He
 
Subject wheat
root hairs
yields
phenotypic plasticity
research
 
Description Root hairs play an important role in absorbing water and nutrients in crop plants. Here we optimized high-throughput root hair length (RHL) and root hair density (RHD) phenotyping in wheat using a portable Dinolite™ microscope. A collection of 24 century wide spring wheat cultivars released between 1911 and 2016 were phenotyped for RHL and RHD. The results revealed significant variations for both traits with five and six-fold variation for RHL and RHD, respectively. RHL ranged from 1.01 mm to 1.77 mm with an average of 1.39 mm, and RHD ranged from 17.08 mm−2 to 20.8 mm−2 with an average of 19.6 mm−2. Agronomic and physiological traits collected from five different environments and their best linear unbiased predictions (BLUPs) were correlated with RHL and RHD, and results revealed that relative-water contents (RWC), biomass and grain per spike (GpS) were positively correlated with RHL in both water-limited and well-watered conditions. While RHD was negatively correlated with grain yield (GY) in four environments and their BLUPs. Both RHL and RHD had positive correlation indicating the possibility of simultaneous selection of both phenotypes during wheat breeding. The expression pattern of TaRSL4 gene involved in regulation of root hair length was determined in all 24 wheat cultivars based on RNA-seq data, which indicated the differentially higher expression of the A- and D- homeologues of the gene in roots, while B-homeologue was consistently expressed in both leaf and roots. The results were validated by qRT-PCR and the expression of TaRSL4 was consistently high in rainfed cultivars such as Chakwal-50, Rawal-87, and Margallah-99. Overall, the new phenotyping method for RHL and RHD along with correlations with morphological and physiological traits in spring wheat cultivars improved our understanding for selection of these phenotypes in wheat breeding.
 
Date 2022-09-01
2023-02-28T08:48:37Z
2023-02-28T08:48:37Z
 
Type Journal Article
 
Identifier Maqbool, S., Saeed, F., Raza, A., Rasheed, A. and He, Z. 2022. Association of root hair length and density with yield-related traits and expression patterns of tarsl4 underpinning root hair length in spring wheat. Plants 11(17):2235. https://hdl.handle.net/10883/22167
2223-7747
https://hdl.handle.net/10568/129082
https://hdl.handle.net/10883/22167
https://doi.org/10.3390/plants11172235
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format application/pdf
 
Publisher MDPI AG
 
Source Plants