Record Details

No mutation effect of 50 Hz sinusoidal magnetic field on beta catenin gene phosphorylation site in N-methyl-N-nitrosourea (MNU) induced colon tumor model

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title No mutation effect of 50 Hz sinusoidal magnetic field on beta catenin gene phosphorylation site in N-methyl-N-nitrosourea (MNU) induced colon tumor model
 
Creator Budak, Metin
Kılıç, Mahmut Alp
Kalkan, Tunaya
Tuncel, Handan
 
Subject Colorectal cancer
Electromagnetic radiation
Single-strand conformation polymorphism (SSCP)
 
Description 941-945
The dysregulation of beta-catenin, a key regulator of cadherin-mediated cell adhesion and crucial for embryonic
development and adult tissue processes, has been implicated in various cancers, including colon cancer. Meanwhile, there
have been longstanding concerns about the potential carcinogenic effects of magnetic fields. In this study, we investigated
the possible relationship between beta-catenin dysfunction and 50 Hz sinusoidal magnetic fields (SMF) using an animal
model of N-methyl-N-nitrosourea (MNU)-induced rat colon tumors. To assess beta-catenin phosphorylation, genomic DNA
was extracted from 58 samples using a commercial extraction kit, and the target gene region corresponding to an important
phosphorylation site of beta-catenin was amplified via polymerase chain reaction (PCR). The amplified samples were
subsequently analyzed using the single-strand conformation polymorphism (SSCP) method to detect any differences
between the experimental groups. Surprisingly, our results revealed no significant differences in beta-catenin gene
phosphorylation sites among the groups. These findings suggest that 50 Hz SMF exposure may not directly impact betacatenin
dysfunction in the context of MNU-induced rat colon tumors. Implications of these results and avenues for further
research are discussed.
 
Date 2023-11-28T07:03:02Z
2023-11-28T07:03:02Z
2023-12
 
Type Article
 
Identifier 0975-1009 (Online); 0019-5189 (Print)
http://nopr.niscpr.res.in/handle/123456789/62965
https://doi.org/10.56042/ijeb.v61i12.4370
 
Language en
 
Publisher NIScPR-CSIR, India
 
Source IJEB Vol.61(12) [December 2023]