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Variability in drought stress-induced physiological, biochemical responses and expression of DREB2A, NAC4 and HSP70 genes in groundnut (Arachis hypogaea L.)

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Relation http://oar.icrisat.org/12324/
https://www.sciencedirect.com/science/article/abs/pii/S0254629921003963
https://doi.org/10.1016/j.sajb.2021.09.025
 
Title Variability in drought stress-induced physiological, biochemical responses and expression of DREB2A, NAC4 and HSP70 genes in groundnut (Arachis hypogaea L.)
 
Creator Kokkanti, R R
Vemuri, H
Gaddameedi, A
Rayalacheruvu, U
 
Subject Groundnut
Drought
 
Description Drought stress is one of the most important factors of physiological stress and the major constraint on crop
productivity which limits plant growth and metabolism. The goal of this work was to study the differential response of groundnut genotypes to drought stress at the physiological, biochemical, and molecular levels during summer in the field. Thirty days old seedlings of six genotypes of groundnut viz. Kadiri 9, Narayani, Dharani, JL24, TPT-3, and Kadiri 6 were subjected to drought stress by withdrawing irrigation for 15 days. The results suggested a significant influence of drought stress on the physiological and biochemical levels in all the groundnut genotypes. A substantial decrease for physiological parameters viz. membrane stability index, chlorophyll stability index, and relative water content was observed under moderate and severe stress conditions compared to control across all genotypes. The high proline accumulating genotypes also exhibited lower lipid peroxidation under all stress periods. In Kadiri 9, Narayani, and Dharani genotypes, the antioxidant enzyme activity of superoxide dismutase, catalase, peroxidase, and glutathione reductase was significantly higher than JL24, TPT-3, and Kadiri 6 genotypes under all stress regimes. Among the genotypes tested, Kadiri 9, Narayani, and Dharani retained higher growth, yield, and seed quality characteristics showing tolerance for drought stress. qPCR analysis revealed stress-responsive existence of the selected genes, heat shock
protein 70 (HSP70), dehydration-responsive element binding-2A (DREB2A), and no apical meristem, ATAF1/2, cup-shaped cotyledon 2 (NAC4) with 30 fold increase in the level of expression in Kadiri 9 compared to Kadiri 6.
 
Publisher Elsevier B. V.
 
Date 2021-10-04
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights cc_by_nc_nd
 
Identifier http://oar.icrisat.org/12324/1/South%20African%20Journal%20of%20Botany_144_448-457_2022.pdf
Kokkanti, R R and Vemuri, H and Gaddameedi, A and Rayalacheruvu, U (2021) Variability in drought stress-induced physiological, biochemical responses and expression of DREB2A, NAC4 and HSP70 genes in groundnut (Arachis hypogaea L.). South African Journal of Botany (TSI), 144. pp. 448-457. ISSN 0254-6299