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Bacterial leaf blight disease of wheat (Pseudomonas syringae pv. syringae) in Syria: importance, characterization, and spread

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Title Bacterial leaf blight disease of wheat (Pseudomonas syringae pv. syringae) in Syria: importance, characterization, and spread
 
Creator Abo Bakr, Abdo
 
Contributor Kassem, Mohammad
Kumari, Safaa
Khatib, Fateh
Husien, Naiem
Nader, Asaad
Moukahel, Abdulrahman
 
Subject seed-borne disease
bacterial bligh
 
Description The quantity and quality of wheat production are affected by a number of biotic and abiotic stresses, including bacterial wheat leaf blight disease caused by Pseudomonas syringae pv. syringae (Pss). Pss is considered one of the most important seed-borne diseases, as it can play an important role in the spread of the disease by seed and agricultural trade. The seriousness of Pss is that there are no specialized bacterial disinfectants, so it is very important to use rapid and accurate methods to detect Pss in order to limit its transmission through infected seeds to healthy fields. Therefore, the aim of the study was to investigate the spread of bacterial wheat leaf blight diseases in wheat-growing areas in Syria and to produce specific antibodies for Pss detection. In 2022, approximately 500 wheat leaf samples showing symptoms suggestive of bacterial infection were collected from 107 fields in the northern, central and coastal areas of Syria. A total of 56 bacterial isolates were selected using a semi-selective medium, and were characterized further using morphological, biochemical (LOPAT), and pathogenicity tests to identify the isolate’s virulence. Results showed that the bacterial wheat leaf blight disease was observed and recorded in 71.2% of the surveyed fields, and 85.7% of the isolates were found to belong to the genus Pseudomonas. A representative Pseudomonas isolates were further tested by polymerase chain reaction (PCR) using specific primers for the syringomycin production gene. Molecular analyses revealed that 12 isolates belong to Pseudomonas syringae pv. syringae (Pss). The bacterial suspension of one Pss isolate (H6), collected from a wheat field in central Syria, was inactivated by formaldehyde treatment and injected into a rabbit. The quality of produced antiserum was evaluated by Dot-blot Immunoassay using homologous and heterologous antigens, and the results obtained revealed that the produced antiserum was able to detect Pss with no cross-reactivity with other bacterial genera (e.g. Xanthomonas).
 
Date 2023-12-12T18:17:57Z
2023-12-12T18:17:57Z
 
Type Conference Paper
 
Identifier https://mel.cgiar.org/reporting/download/hash/2c3eac384b2b9dfdbb5c5b922d13a462
Abo Bakr, A, M. Kassem, S. G. Kumari, F. Khatib, N. Husien, N. Asaad and A. R. Moukahel. 2023. Bacterial leaf blight disease of wheat (Pseudomonas syringae pv. syringae) in Syria: importance, characterization, and spread. Abstracts of papers presented in a regional workshop entitled “Advances in Plant Health Technologies in Healthy Plant Propagation Materials Production and Distribution and its role in Food Security”, Basra – Iraq, 15-16 May 2023. Arab Journal of Plant Protection, 41(2): 216-217. https://doi.org/10.22268/AJPP-41.2.197225
https://hdl.handle.net/20.500.11766/68920
Open access
 
Language ar
en
 
Rights CC-BY-NC-4.0
 
Format PDF
 
Publisher Arab Society of Plant Protection (ASPP)