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Evaluation of DNA extraction methods for molecular traceability in cold pressed, solvent extracted and refined groundnut oils: Evaluation of DNA extraction methods for molecular traceability in cold pressed, solvent extracted and refined groundnut oils

Indian Agricultural Research Journals

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Title Evaluation of DNA extraction methods for molecular traceability in cold pressed, solvent extracted and refined groundnut oils: Evaluation of DNA extraction methods for molecular traceability in cold pressed, solvent extracted and refined groundnut oils
 
Creator KEOTSHEPILE PRECIOUS BOJANG
APARNA KUNA
SREERANGAM NCVL PUSHPAVALLI
SUPTA SARKAR
M SREEDHAR
 
Subject DNA extraction, Food adulteration, Finger printing, Groundnut oil, SSR markers
 
Description Groundnut oil (GNO)/ peanut oil is one of the agro-food products with great economic value and hence an attractive target for adulteration and mislabeling. Simple Sequence Repeats (SSR) are markers of choice for DNA fingerprinting studies as they exhibit high polymorphism due to variable number of repeats. Hence, this study was designed to evaluate and optimized a method for DNA isolation from groundnut oil and study the possibility of using the isolated DNA for molecular traceability using SSR markers. Four methods to isolate DNA from groundnut oil were evaluated. All the four methods were modified CTAB protocols, but differed in procedures forextraction, buffer compositions, amount of oil used and DNA carriers. For molecular traceability of oils, extraction and recovery of DNA from edible oil is a key step, especially in refined oils. A method that employed DNA enrichment prior to extraction with CTAB buffer yielded amplifiable DNA from cold pressed GNO, crude hexaneextracted GNO and refined GNO. The optimized method for isolation of DNA from groundnut oil is simple, efficient, less costly and reproducible when compared to chromatography and spectroscopy based techniques.
 
Publisher Indian Society of Oilseeds Research
 
Date 2023-12-14
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/JOR/article/view/145797
10.56739/jor.v40iSpecialissue.145797
 
Source Journal of Oilseeds Research; Vol. 40 No. Specialissue (2023): Journal of Oilseeds Research
0970-2776
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/JOR/article/view/145797/53164
 
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