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Analysis of antimicrobial resistance in non-typhoidal Salmonella collected from pork retail outlets in Vietnam using whole genome sequencing

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Title Analysis of antimicrobial resistance in non-typhoidal Salmonella collected from pork retail outlets in Vietnam using whole genome sequencing
 
Creator Holohan, N.
Wallat, M.
Thi Hai Yen Luu
Clark, E.
Duong Thi Quy Truong
Sinh Dang-Xuan
Hue Thi Kim Vu
Dung Van Truong
Hoang Tran Huy
Hung Nguyen-Viet
Unger, Fred
Son Thi Thanh Dang
Stabler, R.A.
 
Subject antimicrobial resistance
animal products
swine
 
Description Non-typhoidal salmonella (TS) remains a significant health burden worldwide. In Vietnam, pork accounts for 70% of the total meat consumed, and contamination with Salmonella is high. High levels of antimicrobial resistance (AMR) have emerged among porcine NTS and of particular concern is the emergence of colistin resistance, a “last defense” antibioic against multi-drug resistant (MDR) Gram-negative pathogens. This study aimed to investigate the antibiotic susceptibility of 69 NTS isolates collected from the pork retail outlets and slaughterhouses in Vietnam during 2014 a nd 2018/19. Phenotypic testing and whole genome sequencing was used to assess the serotype and AMR gene profiles of the 69 NTS isolates. Seventeen different serotypes were identified, of which S. enterica subsp enterica serotype Typhimurium was the most common followed by S. ser. Rissen, S. ser. London, S. ser. Anatum, and S. ser. Derby. Phenotype AMR was common with 41 (59.4%) isolates deemed MDR. MDR strains were most common in slaughterhouses (83%) and supermarkets (75%) and lowest in traditional markets (38%) and convenience stores (40%). Colistin resistance was identified in 18 strains (15 resistant, three intermediate) with mcr-1 identified in seven isolates (S. ser. Meleagridis, S. Rissen, S. Derby) and mcr-3 in two isolates (S. Typhimurium). This includes the first mcr positive S. Meleagridis to our knowledge. Surprisingly, boutique stores had high levels (60%) of MDR isolates including 5/20 isolates with mcr-1. This study demonstrates that pork from modern retail stores classed as supermarkets or boutique (with pork claiming to be high quality, traceable, environmentally friendly marketed toward higher income consumers) still contained NTS with high levels of AMR.
 
Date 2022-03-29
2022-04-03T11:21:39Z
2022-04-03T11:21:39Z
 
Type Journal Article
 
Identifier Holohan, N., Wallat, M., Thi Hai Yen Luu, Clark, E., Duong Thi Quy Truong, Sinh Dang-Xuan, Hue Thi Kim Vu, Dung Van Truong, Hoang Tran Huy, Hung Nguyen-Viet, Unger, F., Son Thi Thanh Dang and Stabler, R.A. 2022. Analysis of antimicrobial resistance in non-typhoidal Salmonella collected from pork retail outlets in Vietnam using whole genome sequencing. Frontiers in Veterinary Science 9: 816279.
2297-1769
https://hdl.handle.net/10568/119225
https://doi.org/10.3389/fvets.2022.816279
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 816279
 
Publisher Frontiers Media SA
 
Source Frontiers in Veterinary Science