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Characterization of Pyrenophora tritici-repentis in Tunisia and Comparison with a Global Pathogen Population

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Title Characterization of Pyrenophora tritici-repentis in Tunisia and Comparison with a Global Pathogen Population
 
Creator Laribi, Marwa
Akhavan, Alireza
Ben M’Barek, Sarrah
Yahyaoui, Amor Hassine
Strelkov, Stephen E.
Sassi, Khaled
 
Subject pyrenophora tritici repentis
microsatellites
foliar diseases
wheat
 
Description Pyrenophora tritici-repentis causes tan spot, an important foliar disease of wheat. A collection of P. tritici-repentis isolates from Tunisia, located in one of the main secondary centers of diversification of durum wheat, was tested for phenotypic race classification based on virulence on a host differential set and for the presence of the necrotrophic effector (NE) genes ToxA, ToxB, and toxb by PCR analysis. While races 2, 4, 5, 6, 7, and 8 were identified according to their virulence phenotypes, PCR testing indicated the presence of “atypical” isolates that induced necrosis on the wheat differential ‘Glenlea,’ but lacked the expected ToxA gene, suggesting the involvement of other NEs in the P. tritici-repentis/wheat interaction. Genetic diversity and the P. tritici-repentis population structure were explored further by examining 59 Tunisian isolates and 35 isolates from Algeria, Azerbaijan, Canada, Iran, and Syria using 24 simple sequence repeat markers. Average genetic diversity, overall gene flow, and percentage polymorphic loci were estimated as 0.58, 2.09, and 87%, respectively. Analysis of molecular variance showed that 81% of the genetic variance occurred within populations and 19% occurred between populations. Cluster analysis by the unweighted pair group method indicated that ToxB– isolates grouped together and were distantly related to ToxB+ isolates. Based on Nei’s analysis, the global collection clustered into two distinct groups according to their region of origin. The results suggest that geographic origin and the host specificity imposed by different NEs can lead to differentiation among P. tritici-repentis populations.
 
Date 2022-02-01
2023-04-12T15:03:42Z
2023-04-12T15:03:42Z
 
Type Journal Article
 
Identifier Laribi, M., Akhavan, A., Ben M’Barek, S., Yahyaoui, A. H., Strelkov, S. E. and Sassi, K. 2022. Characterization of pyrenophora tritici-repentis in Tunisia and comparison with a global pathogen population. Plant Disease, 106(2), 464–474
1943-7692
https://hdl.handle.net/10568/129955
https://doi.org/10.1094/PDIS-04-21-0763-RE
 
Language en
 
Rights Copyrighted; all rights reserved
Limited Access
 
Publisher American Phytopathological Society
 
Source Plant Disease