Record Details

Introgression of the QTL qSB11-1TT conferring sheath blight resistance in rice (Oryza sativa) into an elite variety, UKMRC 2, and evaluation of its backcross-derived plants

CGSpace

View Archive Info
 
 
Field Value
 
Title Introgression of the QTL qSB11-1TT conferring sheath blight resistance in rice (Oryza sativa) into an elite variety, UKMRC 2, and evaluation of its backcross-derived plants
 
Creator Hossain, Md. Kamal
Islam, Mohammad Rafiqul
Sundaram, Raman Meenakshi
Bhuiyan, Md. Atiqur Rahman
Wickneswari, Ratnam
 
Subject blight
fungal disease
Rhizoctonia solani
disease resistance
quantitative trait loci
varieties
backcrossing
yield potential
introgression
phenotyping
 
Description Introduction: Sheath blight (SB) is the most damaging fungal disease in rice caused by a soil-borne pathogenic fungus, Rhizoctonia solani Kuhn (R. solani). The disease resistance in rice is a complex quantitative trait controlled by a few major genes. UKMRC2 is a newly developed elite rice variety that possesses high yield potential but is susceptible to sheath blight disease indicating a huge risk of varietal promotion, mass cultivation, and large-scale adoption. The aim of our present study was the development of varietal resistance against R. solani in UKMRC2 to enhance its stability and durability in a wide range of environments and to validate the effects of an SB-resistance QTL on the new genetic background.
Methods: In our study, we developed 290 BC1F1 backcross progenies from a cross between UKMRC2 and Tetep to introgress the QTL qSBR11-1TT into the UKMRC2 genetic background. Validation of the introgressed QTL region was performed via QTL analysis based on QTL-linked SSR marker genotyping and phenotyping against R. solani artificial field inoculation techniques.
Results and Discussion: The QTL qSBR11-1TT was then authenticated with the results of LOD score (3.25) derived from composite interval mapping, percent phenotypic variance explained (14.6%), and additive effect (1.1) of the QTLs. The QTL region was accurately defined by a pair of flanking markers K39512 and RM7443 with a peak marker RM27360. We found that the presence of combination of alleles, RM224, RM27360 and K39512 demonstrate an improved resistance against the disease rather than any of the single allele. Thus, the presence of the QTL qSBR11-1TT has been validated and confirmed in the URMRC2 genetic background which reveals an opportunity to use the QTL linked with these resistance alleles opens an avenue to resume sheath blight resistance breeding in the future with marker-assisted selection program to boost up resistance in rice varieties.
 
Date 2023-01-09
2023-11-04T12:57:12Z
2023-11-04T12:57:12Z
 
Type Journal Article
 
Identifier Hossain, Md Kamal, Mohammad Rafiqul Islam, Raman Meenakshi Sundaram, Md Atiqur Rahman Bhuiyan, and Ratnam Wickneswari. "Introgression of the QTL qSB11-1TT conferring sheath blight resistance in rice (Oryza sativa) into an elite variety, UKMRC 2, and evaluation of its backcross-derived plants." Frontiers in Plant Science 13 (2023): 981345.
1664-462X
https://hdl.handle.net/10568/132721
https://www.frontiersin.org/articles/10.3389/fpls.2022.981345/full
https://doi.org/10.3389/fpls.2022.981345
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Format 14 p.
application/pdf
 
Source Frontiers in Plant Science