Record Details

Survey for legume and cereal viruses in Libya

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Title Survey for legume and cereal viruses in Libya
 
Creator Abukraa, H.
Kumari, Safaa
Bshia, F.
 
Subject molecular characterization
detection
plant viruses
serological diagnosis
barley
faba bean
wheat
lentil
chickpea
grass pea (lathyrus sativus)
 
Description A field survey was conducted during March and April 2010, to identify viral diseases affecting cereal and legume crops in different regions of Libya. A total of 3706 barley and wheat samples were collected randomly in addition to 187 symptomatic samples from 22 barley and 20 wheat fields. Moreover, 34 symptomatic legume samples were collected from two faba bean fields and one field each of of lentil, chickpea, Vicia sativa, Vicia ervillia and Vicia narbonesis. All samples were tested at ICARDA Virology Laboratory by tissue blot immunoassay (TBIA) using specific antibodies. Results of random cereal samples showed that Barley yellow dwarf virus-PAV (BYDV-PAV) was more common (12.6%) in wheat and barley samples, followed by Wheat dwarf virus (WDV) (1.1%). In symptomatic samples, BYDV-PAV was also the most common (45 out of 187 samples tested were infected: 45/187), followed by WDV (23/187), Barley yellow striate mosaic virus (BYSMV) (40/187) and Maize streak virus (MSV) (7/187). In legume fields, Soybean dwarf virus (SbDV) 13 out of 34 samples tested were infected (13/34) was the most common, followed by Bean leafroll virus (BLRV) (12/34), Chickpea chlorotic stunt virus (CpCSV) (5/34) and Beet western yellows virus (BWYV) (4/34). Serological results were confirmed by amplification with polymerase chain reaction (PCR) using specific primers. This is the first report of WDV, MSV and BYSMV on cereal crops, and BLRV, BWYV, SbDV and CpCSV on legume crops in Libya.
 
Date 2022-09-30
2023-01-23T18:50:36Z
2023-01-23T18:50:36Z
 
Type Journal Article
 
Identifier Abukraa, H. S. G. Kumari and F. Bshia. 2022. Survey for legume and cereal viruses in Libya. Arab Journal of Plant Protection, 40(3): 222-230. https://doi.org/10.22268/AJPP-40.3.222230
0255-982X
https://hdl.handle.net/10568/127915
https://dx.doi.org/10.22268/AJPP-40.3.222230
 
Language ar
en
 
Rights CC-BY-4.0
Open Access
 
Format 222-230
application/pdf
 
Publisher Arab Society for Plant Protection
 
Source Arab Journal of Plant Protection