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Expression profiling and functional characterization of miR-192 throughout sheep skeletal muscle development

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Title Expression profiling and functional characterization of miR-192 throughout sheep skeletal muscle development
 
Creator Qian-Jun Zhao
Ye Kang
Hong-Yang Wang
Weijun, Guan
Xiang-Chen Li
Lin Jiang
Xiao-Hong He
Yabin Pu
Han Jianlin
Yue-Hui Ma
 
Subject muscles
sheep
skeletal development
 
Description MicroRNAs (miRNAs) are evolutionarily conserved, small, non-coding RNAs that have emerged as key regulators of myogenesis. Here, we examined the miRNA expression profiles of developing sheep skeletal muscle using a deep sequencing approach. We detected 2,396 miRNAs in the sheep skeletal muscle tissues. Of these, miR-192 was found to be up-regulated in prenatal skeletal muscle, but was down-regulated postnatally. MiR-192 expression also decreased during the myogenic differentiation of sheep satellite cells (SCs). MiR-192 overexpression significantly attenuated SCs myogenic differentiation but promoted SCs proliferation, whereas miR-192 inhibition enhanced SCs differentiation but suppressed SCs proliferation. We found that miR-192 targeted retinoblastoma 1 (RB1), a known regulator of myogenesis. Furthermore, knockdown of RB1 in cultured cells significantly inhibited SCs myogenic differentiation but accelerated SCs proliferation, confirming the role of RB1 in myogenesis. Taken together, our findings enrich the ovine miRNA database, and outline the miRNA transcriptome of sheep during skeletal muscle development. Moreover, we show that miR-192 affects SCs proliferation and myogenic differentiation via down-regulation of RB1.
 
Date 2016-07-25
2016-12-21T13:06:38Z
2016-12-21T13:06:38Z
 
Type Journal Article
 
Identifier Qian Zhao, Ye Kang, Hong-Yang Wang, Wei-Jun Guan, Xiang-Chen Li, Lin Jiang, Xiao-Hong He, Ya-Bin Pu, Jian-Lin Han, Yue-Hui Ma and Qian-Jun Zhao. 2016. Expression profiling and functional characterization of miR-192 throughout sheep skeletal muscle development. Scientific Reports 6: 30281.
2045-2322
https://hdl.handle.net/10568/78439
https://doi.org/10.1038/srep30281
 
Language en
 
Rights CC-BY-4.0
Open Access
 
Publisher Springer Science and Business Media LLC
 
Source Scientific Reports