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Phylogenetic analysis of recent classical swine fever virus isolates (CSFV) from Karnataka, India

Indian Agricultural Research Journals

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Title Phylogenetic analysis of recent classical swine fever virus isolates (CSFV) from Karnataka, India
 
Creator SHIVARAJ, D B
PATIL, S S
RATHNAMMA, D
HEMADRI, D
ISLOOR, S
GEETHA, S
MANJUNATHA REDDY, G B
SHARADA, R
RAHMAN, H
 
Subject 5 UTR
CSF
Classical swine fever
Epidemiology
Karnataka
Phylogenetic analysis
 
Description Classical swine fever (CSF) outbreaks are detected throughout the year. Tissue (spleen and lymph nodes) samples (6) collected from 5 classical swine fever outbreaks in different districts of Karnataka state were subjected to virus isolation in PK–15 cells and virus could be recovered from all the 6 samples. Virus isolation was confirmed by reverse transcription-PCR using primers specific to 5’UTR region and sequenced. Alignment of 150 nucleotides of 5’UTR region with that of 33 published sequences available in Genbank, including 22 Indian CSFV sequences was done to get better epidemiological information on classical swine fever virus (CSFV). Based on the phylogenetic analysis, the isolates from Karnataka could be grouped into subgroup 2.2. Phylogenetic tree confirmed that members of 2.2 subgroup show higher divergence among themselves when compared to other cluster within subgroup 1.1. The study also revealed involvement of 2 different isolates in an outbreak. Most of the outbreaks in our study are due to recent introduction of new stocks of pigs from other places. Even though group 1 viruses are predominant in India, the group 2 viruses are also gradually spreading as confirmed by frequent detection/ isolation of group 2 viruses in the recent years. It appears that subgroup 2.2 viruses are gradually replacing the subgroup 1.1 viruses, indicating the changing epidemiological scenario.
 
 
Publisher Indian Council of Agricultural Research
 
Date 2014-03-07
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/38692
10.56093/ijans.v84i3.38692
 
Source The Indian Journal of Animal Sciences; Vol. 84 No. 3 (2014); 236–239
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/38692/17403
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0