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Omp31 gene based molecular detection of Brucella melitensis from serum samples of goats

Indian Agricultural Research Journals

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Title Omp31 gene based molecular detection of Brucella melitensis from serum samples of goats
 
Creator SINGH, A
GUPTA, V K
KUMAR, A
SINGH, V K
N, SHIVASARANAPPA
 
Subject Brucellosis
Goat
Omp31gene
Serum PCR
 
Description The present study analyses the sensitivity and specificity of OMP31 gene based amplification directly from sera samples. Sero-positive animals (73) in rOmp31 i-ELISA were subjected to Omp31 gene amplification from sera samples and only 40 samples amplified 723kb product suggesting these as true positive animals. When similar test was applied with the sera samples of 16 infected (culture positive) and 20 non-infected (culture negative) goats, all the culture positive animals revealed 723 kb product while none of the culture negative goats revealed amplification. Hence serum based PCR appeared to be 100% sensitive and specific in truly infected animals. Thus serum is the optimal specimen for the diagnosis of brucellosis and leads to simplification of assay and shortens turnaround time.
 
Publisher Indian Council of Agricultural Research
 
Date 2014-03-07
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/38697
10.56093/ijans.v84i3.38697
 
Source The Indian Journal of Animal Sciences; Vol. 84 No. 3 (2014); 251–253
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/38697/17406
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0