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Phenotypic and antigenic characterization of Mycoplasma gallisepticum by RFLP and SDS-PAGE

Indian Agricultural Research Journals

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Title Phenotypic and antigenic characterization of Mycoplasma gallisepticum by RFLP and SDS-PAGE
 
Creator K, VADIVALAGAN
K, SUKUMAR
V, SUDHA RANI
M, SIVACHANDRAN
N, DORAIRAJAN
A, MANICAVASAKA DINAKARAN
 
Subject Mycoplasma gallisepticum
PCR
Phenotypic variation
RFLP
SDS-PAGE
 
Description Phenotypic and antigenic variations among Mycoplasma gallisepticum vaccine 6/85 and field isolates were investigated by SDS-PAGE, RFLP and haemagglutination inhibition test. The SDS-PAGE system followed by photographic analysis showed weak variability between isolates, being the major difference close to the 150 kDa level where a prominent peptide band was detected only in the MG4 isolate. After digestion with Eco R1, two bands of sizes approximately 330 bp and 190 bp could be appreciated only in MG4 field isolate. Hyperimmune serum was used in serological test for this antigenic variability study. There were strong cross reaction between isolates. These studies verified the utility of newer technologies for disease outbreak investigation and the possibility of MG causing disease in layers under the field condition.
 
Publisher Indian Council of Agricultural Research
 
Date 2014-03-07
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/38708
10.56093/ijans.v84i3.38708
 
Source The Indian Journal of Animal Sciences; Vol. 84 No. 3 (2014); 281–283
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/38708/17412
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0