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Effect of vitrification and slow freezing on in-vitro matured prepubertal goat oocytes

Indian Agricultural Research Journals

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Title Effect of vitrification and slow freezing on in-vitro matured prepubertal goat oocytes
 
Creator SINHA, RAJNI
KHARCHE, S D
SINHA, N K
GOEL, A K
JINDAL, S K
SARASWAT, SONIA
 
Subject Cleavage
Cryopreservation
Oocytes
Prepubertal goats
Vitrification
 
Description The present study was conducted to study the post thaw recovery rate of morphologically normal matured oocytes subjected to vitrification and slow freezing method. Oocytes were matured in TCM-199, which comprises sodium pyruvate (0.25 mM), L- glutamine (1 mg/10 ml), gentamycin (50 μg/ml) and supplemented with 10% fetal bovine serum at 38.5°C in CO2 incubator under humidified air. After 27 h of in-vitro maturation oocytes were assigned for freezing, viz. vitrification, slow cooling and control cooling. The percentage of oocytes recovered after thawing was significantly higher for control than those for vitrification (69.67%) and slow freezing (85.00%), respectively. Moreover, the cleavage rate in vitrification, slow freezing and control were 1.14, 3.92 and 13.37%, respectively. Thus, the cleavage rate of oocytes in vitrification and slow freezing method did not differ significantly. However, there was a significant difference in cleavage rates of freezing groups and the control groups.
 
Publisher Indian Council of Agricultural Research
 
Date 2014-03-07
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/38711
10.56093/ijans.v84i3.38711
 
Source The Indian Journal of Animal Sciences; Vol. 84 No. 3 (2014); 289–292
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/38711/17415
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0