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Role of antioxidant additives in the protection of DNA integrity of buck spermatozoa with RAPD assay

Indian Agricultural Research Journals

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Field Value
 
Title Role of antioxidant additives in the protection of DNA integrity of buck spermatozoa with RAPD assay
 
Creator SARASWAT, SONIA
JINDAL, S K
KHARCHE, S D
ROUT, P K
RANJAN, R
PRIYADHARSINI, R
 
Subject Antioxidants
Buck semen
Random amplified polymorphic DNA
 
Description The role of antioxidants (reduced glutathione, ascorbic acid and α-tocopherol) on DNA integrity with the random amplified polymorphic DNA (RAPD) was studied. This assay relies on presence or absence of amplification product on ethidium bromide stained agarose gels. The size of amplification products ranged from 190 to 1,900 base pair. Antioxidants effectively protect sperm DNA from damage. Moreover, random amplified polymorphic DNA technique can be used for the studies of the molecular seminal characteristics of buck semen.
 
Publisher Indian Council of Agricultural Research
 
Date 2014-03-07
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/38713
10.56093/ijans.v84i3.38713
 
Source The Indian Journal of Animal Sciences; Vol. 84 No. 3 (2014); 295–297
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/38713/17417
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0