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Characteristics and freezability of Tharparkar bull semen

Indian Agricultural Research Journals

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Title Characteristics and freezability of Tharparkar bull semen
 
Creator KEDIA, N K
TIWARI, R P
MISHRA, G K
POYAM, M R
PANDEY, A K
NAIR, A K
SAHASRABUDHE, S A
 
Subject Cryopreservation
Seminal attributes
Tharparkar bulls
 
Description The study was undertaken to know the characteristics of various attributes of ejaculates collected from 6 elite pure Tharparkar bulls using artificial vagina in fresh and cryopreserved semen. The mean values for fresh seminal parameters, viz. seminal volume (ml), mass motility (0–5 scale), sperm concentration (millions/ml), individual motility (%), live sperm (%), intact acrosome (%), total sperm abnormalities (%), hypo osmotic swelling test (HOST -%) and cervical mucus penetration test (CMPT – mm) were 4.40 ± 0.24, 3.15 ± 0.12, 995.16 ± 70.99, 71.88 ± 0.99, 67.23± 1.08, 79.65 ± 1.21, 19.87± 0.54, 60.15 ± 1.76 and 31.56 ± 0.96, respectively. Semen volume and sperm concentration significantly differed between bulls within breed. The semen was cryopreserved after extension maintaining 20 million sperm/ straw, filling and sealing in 0.25 ml straw using programmable bio- freezer (IMV). Cryopreserved semen was assessed after 24 h freezing after thawing. Freezing significantly lowered motility (71.88 ± 0.99 vs 51.16 ± 1.08), intact acrosome (79.65 ± 1.21 vs 72.43 ± 1.42), HOST (60.15 ±1.76 vs 55.81 ±1.72) and CMPT (31.56 ± 0.96 vs 26.90 ± 0.91). Significantly higher percentages of sperm abnormalities (19.87 ± 0.54 vs 23.50 ± 0.82) were observed after freezing. Freezing resulted in significant decline in post thaw motility, acrosomal integrity, HOST and CMPT and increase in sperm abnormalities in Tharparkar bull semen.
 
Publisher Indian Council of Agricultural Research
 
Date 2014-04-16
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/39837
10.56093/ijans.v84i4.39837
 
Source The Indian Journal of Animal Sciences; Vol. 84 No. 4 (2014); 382–388
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/39837/17926
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0