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Glycoproteins profiles of placental extract -A step towards development of biomarkers of pregnancy in water buffalo

Indian Agricultural Research Journals

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Title Glycoproteins profiles of placental extract -A step towards development of biomarkers of pregnancy in water buffalo
 
Creator KUMAR, PRADEEP
SAXENA, ABHISHEK
SINGH, S K
AGARWAL, S K
 
Subject Buffalo
Cotyledons
Glycoproteins
 
Description In this study, profiles of glycoproteins of buffalo cotyledons at different stages of pregnancy were studied. First time, WGA lectin affinity chromatography was used to isolate glycoproteins from cotyledons at different stages of gestation in buffalo. The hyper immune sera against the isolated glycoproteins showed no cross reactivity to non- pregnant tissue extract confirming the presence of glycoproteins in samples associated with pregnancy. During early pregnancy, presence of 4 glycoproteins of molecular weights 86, 67, 56 and 51 kDa and out of these glycoproteins, 3 glycoproteins (67, 56 and 51 kDa) were found immunoreactive on western blot analysis. Five glycoproteins of molecular weights 86, 75, 67, 56 and 38 kDa were identified in the mid- and late- stages of pregnancy and all were found immunoreactive. These glycoproteins may be useful for development of RIA/ ELISA based species specific assay for pregnancy diagnosis and monitoring of fetus in buffalo.
 
Publisher Indian Council of Agricultural Research
 
Date 2014-04-16
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/39847
10.56093/ijans.v84i4.39847
 
Source The Indian Journal of Animal Sciences; Vol. 84 No. 4 (2014); 424–426
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/39847/17934
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0