Exploring broilers and native fowls of Andaman and Nicobar Islands as a source of β-lactamase-producing Enterobacteriaceae even with limited anthropogenic activities and docking-based identification of catalytic domains in novel β-lactamase variants
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Title |
Exploring broilers and native fowls of Andaman and Nicobar Islands as a source of β-lactamase-producing Enterobacteriaceae even with limited anthropogenic activities and docking-based identification of catalytic domains in novel β-lactamase variants
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Creator |
Bhowmick S, Pal S, Sunder J, Sujatha T, De AK, Mondal T, Singh AD, Joardar SN, Batabyal K, Dutta TK, Bandyopadhyay S, Tiwari A and Samanta I
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Subject |
Andaman and Nicobar, docking, clonal, ESBL, poultry
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Description |
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Objectives: The present study was conducted to detect the occurrence of b- lactamase and biofilm-producing Escherichia coli, Salmonella, and Klebsiella in broilers and native fowl reared in the Andaman and Nicobar Islands, India. The study also included molecular docking experiments to confirm the nature of the catalytic domains found in the b-lactamase variants obtained and to reveal the clonal relationship of the isolates with human clinical strains from the database. Materials and methods: A total of 199 cloacal swabs were collected from five poultry breeds/varieties (broiler, Vanraja, Desi, Nicobari, and layer) in three districts of the Andaman and Nicobar Islands. E. coli, Salmonella enterica, and Klebsiella pneumoniae were isolated by standard techniques and confirmed by PCR. Phenotypical b-lactamase producers were identified by a double-disc test. The genes (blaCTX, blaSHV, blaTEM, and blaAmpC) were screened, and selected sequences of b-lactamase variants were submitted to DDBJ.Homologymodeling,model validation, and active site identification of dierent b-lactamase variants were done by the SWISS-MODEL. Molecular docking was performed to identify the catalytic domains of the b-lactamase variants. The selected b-lactamase sequences were compared with the Indian ESBL sequences from human clinical strains in NCBI-GenBank. Results: In total, 425 Enterobacteriaceae strains were isolated from the collected samples. Klebsiella pneumoniae (42.58%) was found to be the most prevalent, followed by Salmonella enterica (30.82%) and E. coli (26.58%). The phenotypical antibiogram of all 425 isolates showed the highest resistance against oxytetracycline (61–76%) and the lowest against gentamicin (15– 20%). Phenotypical production of b-lactamase enzymes was observed in 141 (33.38%) isolates. The isolation rate of b-lactamase producing E. coli, Salmonella enterica, and Klebsiella pneumoniae was significantly higher (p Not Available |
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Date |
2023-12-18T13:35:42Z
2023-12-18T13:35:42Z 2023-01-05 |
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Type |
Article
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Identifier |
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Not Available http://krishi.icar.gov.in/jspui/handle/123456789/81069 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Not Available
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