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Perioperative stress prolong post-surgical pain via miR-339-5p targeting oprm1 in the amygdala

Harvard Dataverse (Africa Rice Center, Bioversity International, CCAFS, CIAT, IFPRI, IRRI and WorldFish)

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Title Perioperative stress prolong post-surgical pain via miR-339-5p targeting oprm1 in the amygdala
 
Identifier https://doi.org/10.7910/DVN/1V8QV2
 
Creator Yi Zhu
Mei Sun
Peng Liu
Weidong Shao
Ming Xiong
Bo Xu
 
Publisher Harvard Dataverse
 
Description The decreased expression of mu-opioid receptors (MOR) in the amygdala
may be a key molecular in chronic post-surgical pain (CPSP). It is known that
miR-339-5p expression in the amygdala of a stressed rat model was increased. Analyzed by RNAhybrid, miR-339-5p could target opioid receptor mu 1 (oprm1) which
codes MOR directly. So, the authors hypothesized that miR-339-5p could regulate
the expression of MOR via targeting oprm1 and cause the effects to CPSP.
To simulate perioperative short-term stress, a perioperative stress prolongs
incision-induced pain hypersensitivity without changing basal pain perception
rat model was built. A pmiR-RB-REPORT™ dual luciferase assay was taken to verify
whether miR-339-5p could act on oprm1 as a target. The serum glucocorticoid level
of rats was test. Differential expressions of MOR, GFAP, and pERK1/2 in each group
of the rats’ amygdala were tested, and the expressions of miR-339-5p in each group
of rats’ amygdalas were also measured.
 
Subject Medicine, Health and Life Sciences
Amygdala
Glucocorticoids
MicroRNAs
Models, Animal
Pain Perception
Pain, Postoperative
Physiopathology
Receptors, Opioid, mu
Stress, Physiological
 
Contributor Nahm, Francis