Record Details

Biochemical composition of the ovary and partial characterisation of yolk protein vitellin in Metapenaeus monoceros (Fabricius, 1798)

CMFRI Repository

View Archive Info
 
 
Field Value
 
Relation http://eprints.cmfri.org.in/5866/
 
Title Biochemical composition of the ovary and partial
characterisation of yolk protein vitellin in Metapenaeus
monoceros (Fabricius, 1798)
 
Creator Abraham, Joice
Manisseri, Mary K
 
Subject Biochemical composition
 
Description Yolk protein vitellin in ‘speckled shrimp’ Metapenaeus monoceros was partially characterised
employing polyacrylamide gel electrophoresis (PAGE). There was an increase in the level of
expression of vitellin in the ovary, with the advancement of maturation. The vitellin fraction was
not expressed in immature and spent ovaries. Molecular weight of vitellin was 326 kDa.
Confirmation of the vitellin band by selective staining showed that the yolk protein was a lipoglyco-
carotenoprotein possessing a calcium moiety. A protein fraction with molecular weight
270 kDa, which was a lipoprotein, was expressed in ovaries of all maturity stages as well as in
testis. The major biochemical constituents of mature yolk of M. monoceros on dry weight basis
were protein (59.36±1.18%), lipid (31.23±2.98%) and carbohydrates (2.97±0.81%). Carotenoids
accounted for 0.70±0.81% wet weight of yolk.
 
Publisher CMFRI: Kochi
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/5866/1/a2.pdf
Abraham, Joice and Manisseri, Mary K (2007) Biochemical composition of the ovary and partial characterisation of yolk protein vitellin in Metapenaeus monoceros (Fabricius, 1798). Indian Journal of Fisheries, 54 (3). pp. 299-304.