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CRISPR/Cas9 Editing of Bovine Mammary Epithelial Cells

Harvard Dataverse (Africa Rice Center, Bioversity International, CCAFS, CIAT, IFPRI, IRRI and WorldFish)

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Title CRISPR/Cas9 Editing of Bovine Mammary Epithelial Cells
 
Identifier https://doi.org/10.7910/DVN/YATECZ
 
Creator Moody, Janelle
 
Publisher Harvard Dataverse
 
Description In vitro investigation of bovine lactation processes is limited by a lack of physiologically representative cell models. This deficiency is most evident through the minimal or absent expression of lactation-specific genes in cultured bovine mammary tissues. Primary bovine mammary epithelial cells (pbMECs) extracted from lactating mammary tissue and grown in culture initially express milk protein transcripts at relatively representative levels. However, expression drops dramatically after only three or four passages which greatly reduces the utility of primary cells to model and further investigate lactogenesis.
To investigate the effects of alternate alleles in pbMECs including effects on transcription, we have developed methods to deliver CRISPR-Cas9 gene editing reagents to primary mammary cells, resulting in very high editing efficiencies. We have also found that culturing the cells on an imitation basement membrane composed of Matrigel results in the restoration of a more representative lactogenic gene expression profile and the cells forming three-dimensional structures in vitro.
Here, we present data from four pbMEC lines recovered from pregnant cows and detail the expression profile of five key milk synthesis genes in these MECs grown on Matrigel. Additionally, we describe an optimised method for preferentially selecting CRISPR-Cas9 edited cells conferring a knockout of DGAT1, using fluorescence-activated cell sorting (FACS). The combination of these techniques facilitates the use of pbMECs as a model to investigate the effects of gene introgressions and genetic variation in lactating mammary tissue.
 
Subject Agricultural Sciences
Other
Primary bovine mammary epithelial cells, CRISPR-Cas9, Matrigel, DGAT1, FACS, cell culture
 
Contributor Moody, Janelle