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Figure 2 Data

Harvard Dataverse (Africa Rice Center, Bioversity International, CCAFS, CIAT, IFPRI, IRRI and WorldFish)

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Title Figure 2 Data
 
Identifier https://doi.org/10.7910/DVN/7EKI8P
 
Creator Vining, Kyle
 
Publisher Harvard Dataverse
 
Description 2a. Flow cytometry of bone marrow mononuclear cells (BM) and isolated monocytes (Mo). HLA-DR vs. CD11b+ is gated on live-CD45+CD3- cells, and CD14 vs. CD11b is gated on live-CD45+CD3-CD11b+ cells.
2b. Unsupervised hierarchical clustering of cytokines secreted by monocytes cultured in various matrices. Soft ~ 0.75 kPa, Stiff ~ 7.5 kPa elastic moduli.
2c. Unbiased hierarchical clustering of gene expression of monocytes in matrices from a nanoString myeloid panel.
2d. Clustering of global directed significance scores of gene set analysis. E) Principal component analysis of gene expression with donor 1 and donor 2
2f. Volcano plots of differential gene expression comparing viscous and elastic matrices of elastic modulus, E~7.5 kPa (n=3).
2g. Volcano plot of differential gene expression by nanoString in bone marrow from a cohort of patients with early (n=13) or advanced stage, overt PMF (n=16).
2h. Correlation of normalized gene expression in patients with advanced stage, overt PMF (PMF-3, n=16 biologically independent samples) with monocytes in elastic, stiff matrix (E~7.5 kPa, n=3 biological replicates from a single donor).
 
Subject Engineering
Medicine, Health and Life Sciences
 
Contributor Vining, Kyle