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Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes

Harvard Dataverse (Africa Rice Center, Bioversity International, CCAFS, CIAT, IFPRI, IRRI and WorldFish)

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Title Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes
 
Identifier https://doi.org/10.7910/DVN/TRN1QD
 
Creator Chen, Yu-An
 
Publisher Harvard Dataverse
 
Description The architecture of normal and diseased tissues strongly influences the development and progression of disease as well as responsiveness and resistance to therapy. We describe a tissue-based cyclic immunofluorescence (t-CyCIF) method for highly multiplexed immuno-fluorescence imaging of formalin-fixed, paraffin-embedded (FFPE) specimens mounted on glass slides, the most widely used specimens for histopathological diagnosis of cancer and other diseases. t-CyCIF generates up to 60-plex images using an iterative process (a cycle) in which conventional low-plex fluorescence images are repeatedly collected from the same sample and then assembled into a high-dimensional representation. t-CyCIF requires no specialized instruments or reagents and is compatible with super-resolution imaging; we demonstrate its application to quantifying signal transduction cascades, tumor antigens and immune markers in diverse tissues and tumors. The simplicity and adaptability of t-CyCIF makes it an effective method for pre-clinical and clinical research and a natural complement to single-cell genomics.
 
Subject Medicine, Health and Life Sciences
 
Language English
 
Contributor Arena, Sarah
 
Relation https://www.cycif.org/data/lin-elife-2018/osd-figure2.html#s=0#w=0#g=0#m=-1#a=-100_-100#v=0.5_0.5_0.5#o=-100_-100_1_1#p=Q
https://www.cycif.org/data/lin-elife-2018/osd-figure2.html#s=0#w=0#g=0#m=-1#a=-100_-100#v=0.5_0.5_0.5#o=-100_-100_1_1#p=Q
https://www.cycif.org/data/lin-elife-2018/osd-figure11.html#s=0#w=0#g=0#m=-1#a=-100_-100#v=0.5_0.5_0.5#o=-100_-100_1_1#p=Q