ICAR Research Data Repository for Knowledge Management
Peptide fusion improves prime editing efficiency
Harvard Dataverse (Africa Rice Center, Bioversity International, CCAFS, CIAT, IFPRI, IRRI and WorldFish)
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| Title |
Peptide fusion improves prime editing efficiency
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| Identifier |
https://doi.org/10.7910/DVN/WDOYRZ
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| Creator |
Velimirovic, Minja
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| Publisher |
Harvard Dataverse
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| Description |
Prime editing enables search-and-replace genome editing but is limited by low editing efficiency. We present a high-throughput approach, PepSEq, to measure how fusion of 12,000 85-amino acid peptides derived from human DNA repair-related proteins influences prime editing efficiency. We show that peptide fusion can enhance prime editing, prime-enhancing peptides combine productively, and a top dual peptide-prime editor increases prime editing significantly in multiple cell lines across dozens of target sites
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| Subject |
Medicine, Health and Life Sciences
Prime editing |
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| Contributor |
Velimirovic, Minja
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