Record Details

Replication Data for: Recent sequence variation in probe binding site affected detection of respiratory syncytial virus group B by real-time RT-PCR

Harvard Dataverse (Africa Rice Center, Bioversity International, CCAFS, CIAT, IFPRI, IRRI and WorldFish)

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Title Replication Data for: Recent sequence variation in probe binding site affected detection of respiratory syncytial virus group B by real-time RT-PCR
 
Identifier https://doi.org/10.7910/DVN/B2PT24
 
Creator Kamau, Everlyn
Nokes, David James
 
Publisher Harvard Dataverse
 
Description

This dataset comprises of Nucleotide sequences used to match primers and probes, and for phylogenetic inferences. A total of 168 N and G gene sequences deposited in Genbank with accession numbers from KX775772 - KX775940. This data is freely available. In our analysis, we observed inconsistencies between two routine RSV diagnostic methods applied in our labs, one method utilizes immunofluorescence microscopy and other molecular probes, and set out to determine.




Sequence data cleaning and contig assembly was done using Sequencher program v.5.1; sequence alignment done using MAFFT v.1.8; phylogenetic inferences done in MEGA v1.6; custom BLAST of the primers and probes done using geneious v8.1.8


 
Subject Medicine, Health and Life Sciences
Respiratory Syncytial Virus
Real-Time Mismatches
Probe
 
Contributor Kamau, Everlyn