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Expression of apoptosis related genes in buffalo embryos produced through in vitro fertilization and parthenogenetic activation

Indian Agricultural Research Journals

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Title Expression of apoptosis related genes in buffalo embryos produced through in vitro fertilization and parthenogenetic activation
 
Creator SINGH, M K
SAW, S
SINGH, K P
SAINI, N
KAUSHIK, R
MANIK, R S
PALTA, P
SINGLA, S K
CHAUHAN, M S
 
Subject Apoptosis
Buffalo
Embryo
Parthenogenesis
 
Description In present study immature oocytes were obtained from buffalo slaughterhouse ovaries and were subjected to in vitro maturation (IVM) in TCM-199 + 10% FBS + 5 µg/ml pFSH + 0.81 mM sodium pyruvate + 5% buffalo follicular fluid for 24 h in a CO2 incubator (5% CO2, 90–95% relative humidity) at 38.5°C. The maturer oocytes were used for IVF and the cleaved embryos were cultured for 8 days, while the parthenotes were produced with exposure of 7% ethanol, 6- dimethyl aminopurine and cultured for 8 days in mCR2aa medium. The relative abundance of BCL-XL transcripts in immature, matured oocytes; and embryos produced through IVF i.e. 2-cell, 4-cell, 8-16-cell, morula and blastocyst stage were 25.33±0.90, 12.67±1.20, 37.67±0.90, 30.67±0.30, 23.67±0.90, 18.33±0.90 and 27.00±1.20 respectively, while in parthenogenesis these values were 23.67±0.88, 13.67±1.20, 23.67±1.20, 22.34±0.88, 24.34±0.88, 33.67±0.88, 45.34±1.20 respectively. Relative abundance of BAX transcripts by IVF were 23.0±0.60, 0.33±0.10, 4.00±0.60, 5.00±0.60, 0.37±0.06, 13.0±0.66 and 56.7±0.90; and by parthenonenesis were 22.3±0.90, 0.13±0.03, 13.67±0.90, 14.0±0.60, 15.33±0.90, 64.67±2.20 and 55.0±2.10 respectively. In conclusion, expression pattern of apoptosis related genes revealed that incidence of apoptosis was significantly higher in IVF and parthenogenetically produced buffalo embryos at stages like immature oocytes, morula and blastocyst stage than the early cleavage stage embryos.
 
Publisher Indian Council of Agricultural Research
 
Date 2014-02-12
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/37831
10.56093/ijans.v84i2.37831
 
Source The Indian Journal of Animal Sciences; Vol. 84 No. 2 (2014); 146–151
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/37831/16963
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0