Record Details

Detection of bovine viral diarrhoea virus (BVDV) in peripheral blood mononuclear cells (PBMCs) of BVDV antibody positive Indian cattle following mitogen stimulation

Indian Agricultural Research Journals

View Archive Info
 
 
Field Value
 
Title Detection of bovine viral diarrhoea virus (BVDV) in peripheral blood mononuclear cells (PBMCs) of BVDV antibody positive Indian cattle following mitogen stimulation
 
Creator GUPTA, V
MISHRA, N
RAJUKUMAR, K
BEHERA, S P
DUBEY, P
 
Subject Bovine viral diarrhea virus
BVDV antibody
Cattle
Peripheral blood mononuclear cells
PHA
 
Description The aim of the present study was possible detection of BVDV in peripheral blood mononuclear cells (PBMCs) of antibody positive naturally infected Indian cattle following phytohaemagglutinin A (PHA) stimulation. Of 250 serum samples from cattle in organized dairy firms in India tested by virus neutralization test (VNT), 93 cattle (37.2%) were found positive for BVDV neutralizing antibodies. The PBMCs from BVDV-immune cattle were stimulated with 5µg/ml of PHA for 72 h prior to BVDV RNA detection by RT-PCR or infectious virus detection by virus isolation. Of the 93 BVDV immune field cattle tested positive, BVDV RNA was detected only in PHA stimulated PBMCs of 3 cattle, while no infectious BVDV could be detected by virus isolation. This is for the first time, that BVDV RNA was detected following mitogen (PHA) stimulation of PBMCs in BVDV antibody positive Indian cattle.
 
Publisher Indian Council of Agricultural Research
 
Date 2014-02-12
 
Type info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
 
Format application/pdf
 
Identifier https://epubs.icar.org.in/index.php/IJAnS/article/view/37833
10.56093/ijans.v84i2.37833
 
Source The Indian Journal of Animal Sciences; Vol. 84 No. 2 (2014); 156–158
2394-3327
0367-8318
 
Language eng
 
Relation https://epubs.icar.org.in/index.php/IJAnS/article/view/37833/16965
 
Rights Copyright (c) 2014 The Indian Journal of Animal Sciences
https://creativecommons.org/licenses/by-nc-sa/4.0