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Induction of Triploidy in Indian Edible Oyster Crassostrea madrasensis (Preston) Using 6-Dimethylaminopurine

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Relation http://eprints.cmfri.org.in/5739/
http://www.asianfisheriessoceity.org
 
Title Induction of Triploidy in Indian Edible Oyster Crassostrea madrasensis (Preston) Using 6-Dimethylaminopurine
 
Creator Thomas, P C
Mallia, Jyothi V
Muthiah, P
 
Subject Induced Breeding
Edible oyster
 
Description Triploidy was induced in the Indian edible oyster Crassostrea madrasensis, by treating the newly fertilized
eggs with 6-Dimethylaminopurine.The effect of different concentrations and durations of treatment on
triploidy induction and survival of embryos were examined. The optimum condition was found to be 100 μM
concentration of 6-DMAP for 8 minutes when 50% of the freshly fertilized eggs have extruded the first polar
bodies. This yielded 67±1.7 % of triploid on the first day and 62±1.5% on the D-stage larvae as determined by
examination of the metaphase chromosomes. Survivability among the different treatment groups at D stage were
not significantly different. This is the first demonstration of induction of triploidy in Crassostrea madrasensis
using 6-DMAP and since edible oyster farming is becoming increasingly popular in India, application of this
technique in the production of culture stock assumes importance.
 
Publisher Asian Fisheries Soceity; Malaysia
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Identifier http://eprints.cmfri.org.in/5739/1/2.pdf
Thomas, P C and Mallia, Jyothi V and Muthiah, P (2006) Induction of Triploidy in Indian Edible Oyster Crassostrea madrasensis (Preston) Using 6-Dimethylaminopurine. Asian Fisheries Science, 19 (1). pp. 15-20.