Induction of Triploidy in Indian Edible Oyster Crassostrea madrasensis (Preston) Using 6-Dimethylaminopurine
CMFRI Repository
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Relation |
http://eprints.cmfri.org.in/5739/
http://www.asianfisheriessoceity.org |
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Title |
Induction of Triploidy in Indian Edible Oyster Crassostrea madrasensis (Preston) Using 6-Dimethylaminopurine
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Creator |
Thomas, P C
Mallia, Jyothi V Muthiah, P |
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Subject |
Induced Breeding
Edible oyster |
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Description |
Triploidy was induced in the Indian edible oyster Crassostrea madrasensis, by treating the newly fertilized eggs with 6-Dimethylaminopurine.The effect of different concentrations and durations of treatment on triploidy induction and survival of embryos were examined. The optimum condition was found to be 100 μM concentration of 6-DMAP for 8 minutes when 50% of the freshly fertilized eggs have extruded the first polar bodies. This yielded 67±1.7 % of triploid on the first day and 62±1.5% on the D-stage larvae as determined by examination of the metaphase chromosomes. Survivability among the different treatment groups at D stage were not significantly different. This is the first demonstration of induction of triploidy in Crassostrea madrasensis using 6-DMAP and since edible oyster farming is becoming increasingly popular in India, application of this technique in the production of culture stock assumes importance. |
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Publisher |
Asian Fisheries Soceity; Malaysia
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Date |
2006
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Language |
en
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Identifier |
http://eprints.cmfri.org.in/5739/1/2.pdf
Thomas, P C and Mallia, Jyothi V and Muthiah, P (2006) Induction of Triploidy in Indian Edible Oyster Crassostrea madrasensis (Preston) Using 6-Dimethylaminopurine. Asian Fisheries Science, 19 (1). pp. 15-20. |
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